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园艺学报 ›› 2007, Vol. 34 ›› Issue (4): 991-994.

• 观赏植物 • 上一篇    下一篇

乐昌含笑组织培养过程中根的诱导

吴月燕*; 刘秀莲;汪财生   

  1. (浙江万里学院生物与环境学院, 浙江宁波315100)
  • 收稿日期:2007-03-23 修回日期:2007-05-30 出版日期:2007-08-25 发布日期:2007-08-25

Root Induction During Tissue Culture of Michelia chapensis Dandy

WU Yue-yan*; LIU Xiu-lian;WANG Cai-sheng
  

  1. (College of Biology and Environment, Zhejiang Wanli University, Ningbo, Zhejiang 315100, China )
  • Received:2007-03-23 Revised:2007-05-30 Online:2007-08-25 Published:2007-08-25

摘要: 以乐昌含笑为材料, 采用6 - 苄氨基嘌呤(6-BA) 、萘乙酸(NAA) 、吲哚丁酸( IBA) 三因
素二次正交旋转组合设计, 对组织培养过程中根的诱导进行了3种生长调节剂不同配比的对比试验。结果
表明: 在23个组合中, 较低6-BA、NAA和IBA的浓度能促进乐昌含笑根的诱导和生长; 外植体在1/2MS
+ 6-BA 0.40 mg·L-1 + IBA 0.60 mg·L-1 +NAA 0.80 mg·L-1培养基上出根率和生根数分别达到50%和
1.5条以上, 高于其它处理, 而且根系生长良好。

关键词: 乐昌含笑, 根, 诱导, 生长调节剂

Abstract: Effects of 6-BA, NAA and IBA alone or combination at various concentrations on the root in
duction of Michelia chapensis Dandy during tissue culture were investigated. The results showed that low con
centrations of 6-BA, NAA and IBA were suitable for the root induction and growth of Michelia chapensis Dan
dy. The root formation rate wasmore than 50% , while the root number of each p lantwasmore than 1.5 with
grown p lantletswhen the exp lantswere transferred to the 1/2MS medium containing 0.40 mg·L-1 6-BA and
0.60 mg·L-1 IBA and 0.80 mg·L-1 NAA. Meanwhile, itwas observed that the root developed well.

Key words: Michelia chapensis Dandy, Root, Inducement, Plant growth regulator