脱锻炼期间白皮松针叶的电阻抗图谱参数和生理指标的相关性

园艺学报 ›› 2009, Vol. 36 ›› Issue (6): 891-897.

脱锻炼期间白皮松针叶的电阻抗图谱参数和生理指标的相关性

董胜豪¹;张　钢²*;卻书鹏²;祝　良²;靳秀梅²

(¹河北农业大学园艺学院, 河北保定071001; ²北京十三陵林场苗圃, 北京102200)

收稿日期:2008-10-08 修回日期:2009-05-18 出版日期:2009-06-25 发布日期:2009-06-25
通讯作者: 张　钢

The Relationship Between Parameters of Electrical Impedance Spectroscopyand Some Physiological Parameters in Pinus bungeana Zucc. Needles DuringDehardening

DONG Sheng-hao¹, ZHANG Gang¹*, QUE Shu-peng², ZHU Liang², and JIN Xiu-mei²

(¹College of Horticulture, AgriculturalUniversity of Hebei, Baoding, Hebei 071001, China;²The Nursery in Beijing Ming Tombs Forestry Center, Beijing 102200, China)

Received:2008-10-08 Revised:2009-05-18 Online:2009-06-25 Published:2009-06-25
Contact: ZHANG Gang

摘要/Abstract

摘要： 2007年3—5月, 对北京十三陵苗圃的北京蟒山、山西孝义和甘肃两当3个种源的9年生白皮
松( Pinus bungeana Zucc. ) 针叶进行了电阻抗图谱( EIS) 分析, 测定其未冷冻针叶的EIS参数, 以及超氧化物歧化酶( SOD) 活性、过氧化物酶( POD) 活性、丙二醛(MDA) 含量、可溶性蛋白质、脯氨酸和干物质含量等生理指标的变化, 比较脱锻炼期间不同种源白皮松针叶的EIS参数和生理指标之间的相关性。结果表明: (1) 脱锻炼期间未冷冻针叶的EIS参数β和胞外电阻率r_e与针叶的脯氨酸含量之间存在负相关;细胞膜时间恒量τ_m与针叶的可溶性蛋白质含量存在正相关; (2) 胞内电阻率r_i与MDA, τ_m与干物质含量的变化趋势相同, 而r_e和β在脱锻炼前期与可溶性蛋白质含量的变化趋势类似; (3) 冷冻处理后用τ_m和r_e求出的抗寒性与脯氨酸含量相关。

关键词: 白皮松, 脱锻炼, 生理指标, 电阻抗图谱

Abstract: From March to May 2007, the electrical impedance spectroscopy ( EIS) was analyzed on one-year-old needles of Pinus bungeana Zucc. in a 9-year provenance field trial at the Nursery in Beijing MingTombs Forestry Center with provenances of Mangshan in Beijing, Xiaoyi in Shanxi and Liangdang in Gansu.
The activities of superoxide dismutase ( SOD ) and peroxidase ( POD ) , the contents of malondialdehyde(MDA) , soluble proteins and proline, and dry weight percentage in needleswere tested with different provenances. The relations between parameters of the EIS and tested physiological parameters were analyzed. The results indicated that (1) during dehardening, EIS parameters β and specific extracellular resistance r_ein nonfrost-exposed needles correlated negatively with the content of proline; and there was a positive relation between the membrane time constant τ_mand the content of soluble protein; (2) Similar changing patterns were found between the specific intracellular resistance r_iand the content of MDA; Between the τ_mand the percentage of dry weight, and between r_e , β and soluble proteins in the early stage of dehardening; (3) The frost hardiness assessed by r_e and τ_m after artificial freezing treatment correlated to the content of proline.

Key words: Pinus bungeana Zucc., dehardening, physiological parameter, electrical impedance spectroscopy

中图分类号:

董胜豪;张　钢;卻书鹏;祝　良;靳秀梅. 脱锻炼期间白皮松针叶的电阻抗图谱参数和生理指标的相关性[J]. 园艺学报, 2009, 36(6): 891-897.

DONG Sheng-hao;ZHANG Gang;QUE Shu-peng;ZHU Liang;and JIN Xiu-mei. The Relationship Between Parameters of Electrical Impedance Spectroscopyand Some Physiological Parameters in Pinus bungeana Zucc. Needles DuringDehardening[J]. ACTA HORTICULTURAE SINICA, 2009, 36(6): 891-897.

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