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园艺学报 ›› 2008, Vol. 35 ›› Issue (6): 909-916.

• 综述 • 上一篇    下一篇

花色素苷生物合成与分子调控研究进展

张 龙1;李卫华1;姜淑梅3;朱根发1;王碧青1;李洪清2*   

  1. 1广东省农业科学院花卉研究所,广州 510640;2华南师范大学生命科学院,广州 510631;3中国科学院南海海洋研究所,广州 510301)
  • 收稿日期:2008-03-03 修回日期:2008-05-19 出版日期:2008-06-25 发布日期:2008-06-25
  • 通讯作者: 李洪清

Progress of Molecular Basis of Biosynthesis and Transcriptional Regulation of Anthocyanins

ZHANG Long1, LI Wei-Hua1, JIANG Shu-mei3, ZHU Gen-fa1, WANG Bi-qing1, and LI Hong-qing2*   

  1. (1Floriculture Research Institute of Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;2Guangdong Key Laboratory of Biotechnology for Plant Development, The College of Life Science, South China Normal University, Guangzhou 510631, China;3South China Sea Institute of Oceanlology, Chinese Academy of Sciences, Guangzhou 510301, China)
  • Received:2008-03-03 Revised:2008-05-19 Online:2008-06-25 Published:2008-06-25
  • Contact: LI Hong-qing

摘要: 花色素苷是决定植物花色的主要色素,其生物合成是目前研究得最为清楚的植物次生代谢途径之一。花色素苷的生物合成主要由于F3H,F3'H和F3'5'H三个关键酶的作用形成3个分支,最终分别生成橙色到砖红色的天竺葵素糖苷,红色的矢车菊素糖苷和蓝色到紫色的飞燕草素糖苷,因此, F3'H,F3'5'H和DFR基因是利用遗传转化引入花卉植物原本缺乏的花色代谢途径的关键基因。花色素苷合成结构基因的转录调控是目前研究的热点,对结构基因的转录进行调控的转录因子主要包括两大类相互作用的因子-bHLH和MYB类转录因子,花色素苷合成的转录调控机理的基本模式,包括bHLH和MYB类因子之间的相互作用,以及它们对结构基因顺式元件的识别和结合已经阐述的比较清楚。另外,对于一些处于bHLH和MYB上游的, WD40类因子和光敏色素的研究开启了从信号传导到花色素苷合成的整个调控过程的研究。

关键词: 花色素苷, 生物合成, 转录调控

Abstract: Anthocyanins are one of the main determinants of flower colors. The biosynthesis pathway of anthocyanins is one of the most extensively studied plant secondary metabolism. By the catalyzed of F3H, F3'H and F3'5'H, the biosynthesis of anthocyanins branched three pathway to form pelargonidin (orange to brick red), cyanidin (pink to red) and delphinidin (purple to blue) separately. DFR, F3'H and F3'5'H are the most important genes for introducing new metabolic branches by genetic transformation to plants that lack some kinds of flower pigments. The research on biosynthesis of anthocyanins has been focused on the transcriptional regulation of structural genes in latest years. The transcriptional factors involved in biosynthesis of anthocyanins mainly include two kinds of protein families-bHLH and R2R3-MYB. The basic model of transcriptional regulation of biosynthesis of anthocyanins, including the interaction between bHLH and R2R3-MYB factors and the recognizing and binding of these factors to the cis-elements of structural genes, has been set up. Additionally, the study on the WD40s and phytochromes that located upstream of bHLH and R2R3-MYB factors in the transcriptional regulation system originates the research of the whole signal pathway from the stimulation of environmental factors to biosynthesis of anthocyanins.

Key words: anthocyanidin, biosynthesis, transcription regulation

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