核桃离体培养和植株再生

园艺学报 ›› 2009, Vol. 36 ›› Issue (6): 867-872.

核桃离体培养和植株再生

樊　靖^{1, 2};刘庆忠²;张俊林^{2, 3}; 秦　岭⁴;陈　新²;王　锦^1*

(¹西南林学院园林学院, 昆明650224; ²山东省果树研究所, 山东泰安271000;³浙江森禾种业股份有限公司, 杭州310012; ⁴北京农学院植物科学技术系, 北京102206)

收稿日期:2008-12-26 修回日期:2009-03-31 出版日期:2009-06-25 发布日期:2009-06-25
通讯作者: 王　锦

In Vitro Culture and Plant Regeneration of Juglans regia L.

FAN Jing^1,2,LIU Qing^_-zhong²,ZHANG Jun-lin^2,3,QIN L ing⁴, CHEN Xin², and WANG Jin^1*

(¹Faculty of Landscape A rchitecture, Southwest Forestry College, Kunm ing 650224, China; ²Shandong Institute of Pom ology,Tai'an, Shandong 271000, China; ³Zhejiang Senhe Seed Co. , LTD, Hangzhou 310012, China; ⁴Departm ent of Plant Science and Technology, B eijing University of Agriculture, B eijing 102206, China)

Received:2008-12-26 Revised:2009-03-31 Online:2009-06-25 Published:2009-06-25
Contact: WANG Jin

摘要/Abstract

摘要：

以5个核桃品种(株系) 10年生植株的茎段为外植体, 进行了离体培养和植株再生的研究。结果表明, 附加2 g·L^-1聚乙烯吡咯烷酮的DKW + 6-BA 1.0 mg·L^-1 + IBA 0.01～0.10 mg·L ^-1是诱导茎段萌发最适宜的培养基, 5个品种(株系) 间的萌发率存在差异; DKW + 6-BA 110 mg·L^-1 + IBA 0.01mg·L^-1 + 蔗糖40 g·L^-1是最适宜的增殖培养基; 采用二步生根法最高生根率为41.67% , 生根试管苗移栽于草炭土∶河沙= 1 ∶1的基质中, 控制温度20～30 ℃, 保湿20 d, 期间遮荫, 40 d后成活率95%。

关键词: 核桃, 茎段, 组织培养, 聚乙烯吡咯烷酮

Abstract: A study was conducted on in vitro culture and plant regeneration of Juglans regia L. with the nodal segments or apical buds excised from the newly-grown shoots of the ten-year-old of five elites maternal plants. Results showed that the DKW supp lemented with 6-BA 110 mg·L^-1+ IBA 0.01 - 0.10 mg·L^-1and polyvinylpyrrolidone 2 g·L^-1was optimal for segments breaking, five genotypes exhibited differences;While the DKW with 6-BA 1.0 mg·L^-1+ IBA 0.01 mg·L^-1+ 40 g·L^-1sucrose was op timal for the tube-shoot multip lication; The highest rooting frequencywas 41167% by using two step smethod. The plantlets with roots were transp lanted into the substrate containing peat and sand (1 ∶1) , At 20 - 30 ℃ for 20 days, with the survival 95% after 40 days’transplanting.

Key words: walnut, Juglans regia L., stem segment, tissue culture, polyvinylpyrrolidone

中图分类号:

樊　靖;;刘庆忠;张俊林;;秦　岭;陈　新;王　锦. 核桃离体培养和植株再生[J]. 园艺学报, 2009, 36(6): 867-872.

FAN Jing;LIU Qing_-zhong;ZHANG Jun-lin;QIN L ing;CHEN Xin;and WANG Jin. In Vitro Culture and Plant Regeneration of Juglans regia L.[J]. ACTA HORTICULTURAE SINICA, 2009, 36(6): 867-872.

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