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园艺学报 ›› 2017, Vol. 44 ›› Issue (11): 2186-2194.doi: 10.16420/j.issn.0513-353x.2017-0052

• 研究报告 • 上一篇    下一篇

可降解螯合剂对草坪植物高羊茅发育及生理的影响

王思予,多立安*,赵树兰*   

  1. 天津师范大学生命科学学院,天津市动植物抗性重点实验室,天津 300387
  • 出版日期:2017-11-25 发布日期:2017-11-25

Effects of Biodegradable Chelator on Growth and Physiology of Festuca arundinacea Seedlings

WANG Siyu,DUO Li’an*,and ZHAO Shulan*   

  1. College of Life Sciences,Tianjin Key Laboratory of Animal and Plant Resistance,Tianjin Normal University,Tianjin
    300387,China
  • Online:2017-11-25 Published:2017-11-25

摘要:

以盆栽高羊茅为试验材料,研究了土壤中添加不同浓度(3、6、9、12 和15 mmol · kg-1)生
物可降解螯合剂谷氨酸二乙酸四钠(Glutamic acid N, N-diacetic acid tetra sodium salt,GLDA)和亚氨基二
琥珀酸四钠盐(Iminodisuccinic acid sodium salt,IDS)处理对种子萌发和幼苗发育及生理的影响。结果表
明,GLDA 和IDS 处理对高羊茅种子萌发有一定的抑制作用,并且高浓度的抑制作用更强。对高羊茅地
上生物量的影响表现为低浓度促进,高浓度抑制,6 mmol · kg-1 处理生物量达到最大;而所有螯合剂处理
均显著抑制了根系的生长。与对照相比,低浓度的螯合剂(3、6 和9 mmol · kg-1)对叶绿素和类胡萝卜素
含量没有显著影响,但高浓度处理显著降低了其含量。螯合剂对SOD 和CAT 活性的影响呈现先增加后
降低的趋势,而POD 活性和MDA 含量则随螯合剂浓度的增加而升高,表明添加螯合剂对高羊茅构成了
逆境胁迫。鉴于此,在两种螯合剂应用于土壤重金属修复时,以低浓度(6 mmol · kg-1)施加为宜,并且
可以考虑在植物收获前几天施加或分次加入。

关键词: 草坪植物, 可降解螯合剂, GLDA, IDS, 生理

Abstract:

Pot experiment was conducted to investigate the effects of biodegradable chelators–
Glutamic acid N, N-diacetic acid tetra sodium salt(GLDA)and iminodisuccinic acid sodium salt(IDS)
added at different concentrations(3,6,9,12 and 15 mmol · kg-1)on seed germination,growth and
physiology of Festuca arundinacea Schreb. seedlings. The results showed that the addition of GLDA and
IDS inhibited seed germination of F. arundinacea,especially at high concentrations. The addition of
GLDA and IDS at low concentrations increased aboveground biomass , but reduced it at high
concentrations. The highest aboveground biomass was found at the chelator treatment of 6 mmol · kg-1,
which was significantly higher than those in the other chelator treatments. The addition of GLDA and IDS inhibited root growth of F. arundinacea,especially at high concentrations. Compared with the control,
chelators at low concentrations(3,6 and 9 mmol · kg-1)had no significant effects on chlorophyll and
carotenoid contents. However,chelators at high concentrations significantly decreased chlorophyll and
carotenoid contents. The activities of SOD and CAT increased and then decreased with the increase in
chelator concentration,while the POD activity and MDA content increased constantly with the increase in
chelator dosage. The results indicated that the addition of GLDA and IDS may form stress for F.
arundinacea,and the plants could develop resistance to the stress by increasing the activities of antioxidant
enzymes. Thus,when GLDA and IDS are applied in remediation of soil polluted by heavy metals,they
should be applied at a low concentration(6 mmol · kg-1)and should be added several days before plants are
harvested or added at a low dosage in multiple times.

Key words: turfgrass, biodegradable chelator, GLDA, IDS, physiology