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园艺学报 ›› 2017, Vol. 44 ›› Issue (11): 2117-2125.doi: 10.16420/j.issn.0513-353x.2017-0057

• 研究论文 • 上一篇    下一篇

乙烯与脂氧合酶在番茄果实香气合成中的作用

梁馨元,郭星秀,齐红岩*   

  1. 沈阳农业大学园艺学院,设施园艺省部共建教育部重点实验室,环渤海湾地区设施蔬菜优质高效生产协同创新中
    心,沈阳 110866
  • 出版日期:2017-11-25 发布日期:2017-11-25

Role of Lipoxygenase and Ethylene in Tomato Fruit Aroma Synthesis

LIANG Xinyuan,GUO Xingxiu,and QI Hongyan*   

  1. College of Horticulture,Shenyang Agricultural University,Key Laboratory of Protected Horticulture,Ministry of
    Education,Collaborative Innovation Center of Protected Vegetable Surround Bohai Gulf Region,Shenyang 110866,China
  • Online:2017-11-25 Published:2017-11-25

摘要:

通过对番茄成熟突变体和野生型果实香气物质、乙烯释放量、氢过氧化物裂解酶(HPL)、
脂氧合酶(LOX)活性及基因表达的测定,明确LOX 与乙烯在番茄果实香气物质合成中的作用。以番茄
成熟突变体rin、nor、cnr、nr(均为乙烯合成缺陷突变体)、hp-1(高色素突变体,乙烯生成正常)和野
生型Ailsa Craig(AC)为试材,分别在果实破色期(0 d)、破色3 d(3 d)和破色7 d(7 d)取果实,测
定香气物质、乙烯释放量、LeHPL 基因表达、LOX 活性和TomloxC 基因表达。结果表明,随着果实的成
熟,4 种乙烯合成突变体果实中的香气物质种类和含量与野生型AC 相比均减少,但突变体hp-1 果实中
香气物质与AC 无显著差异;rin、nor、cnr 突变体中不产生乙烯,nr 中产生少量乙烯,hp-1 和AC 果实
中能正常生成乙烯;除AC 果实外,突变体rin、nor、cnr 果实中LOX 酶活性和TomloxC 基因表达水平均
较低,而在hp-1 和nr 破色7 d 果实中酶活性和基因表达量均最高;LeHPL 表达量在AC 和hp-1 果实中随
果实成熟显著增加,在nor 破色7 d 显著高于破色期(0 d)和破色3 d 的,而在cnr、rin 和nr 破色7 d 均
显著下降。在乙烯合成缺陷突变体果实中香气物质种类和含量较野生型减少,且不能合成乙烯或合成少
量乙烯,同时LOX 酶活性降低,TomloxC 及LeHPL 表达下降,表明番茄果实香气物质合成的LOX 途径
是依赖乙烯的过程。

关键词: 番茄, 突变体, 脂氧合酶, 乙烯, 香气

Abstract:

In order to acquire a better insight into the ethylene and lipoxygenase control of flavour
compound generation and links between these metabolites and the central regulators of ripening,five
pleiotropic mutant tomato lines were subjected to volatile metabolite profiling in comparison with
wild-type Ailsa Craig(AC). Five tomato mature mutants rin,nr,nor,cnr,hp-1 and wild type AC were
sampled at breaker color(0 d),three days after breaker(3 d)and seven days after breaker(7 d),
respectively. Aroma,ethylene and gene expression of hydroperoxide lyase(HPL),lipoxygenase(LOX)
activity and gene expression of TomloxC were investigated. With fruits growth and development,compared with the control,the aroma compounds in the mutant fruits were decreased. LOX enzyme activity and gene
expression of TomloxC were lower in rin,nor,cnr fruits than that in wild-type AC fruits. However,
expression of TomloxC were significantly increased in the hp-1 fruits and nr fruits at seven days after
breaker. The aroma compounds in ripening mutants of tomato were less than those in wild type,and they
can’t synthesize ethylene or synthesize small amounts of ethylene. Along with fruits growth and
development,the expression of LeHPL in AC and hp-1 were increased,seven days after breaker were
significantly higher than breaker and three days after breaker in the nor fruits,in rin and nr fruits were
significantly decreased at seven days after breaker. Above all,in the mutants lack of ethylene,the aroma
contents and types were lower than those in wild type,the ethylene synthesis were inhibited,LOX activity
and gene expression of LeHPL,TomloxC were decreased. Those results indicated that LOX was regulated
by ethylene in aroma volatiles synthesis of fruits.

Key words: tomato, mutant, lipoxygenase, ethylene, aroma volatile