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园艺学报 ›› 2017, Vol. 44 ›› Issue (7): 1397-1404.doi: 10.16420/j.issn.0513-353x.2016-0862

• 研究报告 • 上一篇    下一篇

过表达香樟CcCBFb 的烟草非生物胁迫抗性增强

张佳佳,朱逢玲,焦晓琳,李勇鹏,王建格,周 婵,杜 丽*   

  1. 南阳师范学院生命科学与技术学院,河南南阳 473000
  • 出版日期:2017-07-25 发布日期:2017-07-25

Overexpression of CcCBFb from Cinnamomum camphora Confers Multiple Stress Tolerance to Tobacco

ZHANG Jiajia,ZHU Fengling,JIAO Xiaolin,LI Yongpeng,WANG Jiange,ZHOU Chan,and DU Li*   

  1. School of Life Science and Technology,Nanyang Normal University,Nanyang,Henan 473000,China
  • Online:2017-07-25 Published:2017-07-25

摘要:

为探究香樟CcCBFb 基因增强植株非生物胁迫抗性的功能,通过农杆菌介导法将该基因转
入烟草中,经PCR 和半定量RT-PCR 技术鉴定阳性转基因株系后,对获得的T1 代转基因无性系(T2 和
T4 株系)以及野生型(WT)进行干旱(0、150、300 和450 mmol · L-1 的甘露醇)、高盐(0、100、200
和300 mmol · L-1 的NaCl)、4 ℃低温、–4 ℃冰冻胁迫处理,结果显示:转基因烟草在干旱和高盐胁迫
下,幼苗的存活率均高于野生型;经4 ℃低温处理后,转基因烟草植株的脯氨酸和可溶性糖含量均显著
高于野生型植株,而丙二醛(MDA)含量均低于野生型植株;经–4 ℃的冰冻处理6 h 后,野生型和转
基因T4 株系植株叶片均出现不同程度的萎蔫,而转基因T2 株系植株未出现不良现象。由此可见,过表
达CcCBFb 基因不但能够增强烟草的抗旱和抗盐性,而且能够显著增强抗寒性。

关键词: 香樟, CcCBFb, 烟草, 非生物胁迫

Abstract:

In order to explore the function of CcCBFb gene from Cinnamomum camphora,the effect
of over-expression of this gene on abiotic stress resistance improvement is assessed by using transgenic
tobacco. With the identification of positive transgenic tobacco by PCR and semi-quantitative RT-PCR
technique,T1 clonal transgenic tobacco(Line 2 and Line 4)and wild type(WT)would be treated under
the drought,salinity,low temperature and freezing stress. The results showed that survival rate of
transgenic tobacco(Line 2 and Line 4)seedling was better to wild tobacco under drought,salinity stress.
The proline and soluble sugar content of transgenic tobacco plants(Line 2 and Line 4)was higher than
wild tobacco but the content of MDA was lower than wild tobacco under low temperature(4 ℃)treatment.
WT and Line 4 had the different degree wilting but the Line 2 had a good growth status under the freezing
treatment (–4 ℃)with 6 h. So,the overexpression of CcCBFb from camphor tree conferred tobacco plants enhanced the tolerance to cold,drought and salinity stress.

Key words: Cinnamomum camphora, CcCBFb, tobacco, abiotic stress