https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2017, Vol. 44 ›› Issue (7): 1379-1387.doi: 10.16420/j.issn.0513-353x.2016-0932

• 研究报告 • 上一篇    下一篇

甜瓜CmGnT 基因的克隆及非生物胁迫下的表达分析

张瑞腾1,2,付秋实1,张 磊3,李双桃3,郭仰东3,张 红4,李灵芝2,李海平2,*,王怀松1,*   

  1. 1 中国农业科学院蔬菜花卉研究所,北京 100081;2 山西农业大学园艺学院,山西太谷 030801;3 中国农业大学园
    艺学院,北京 100193;4 新疆农业科学院哈密瓜研究中心,乌鲁木齐 830091
  • 出版日期:2017-07-25 发布日期:2017-07-25

Cloning and Expression Analyzing of CmGnT Gene in Melon Under Abiotic Stresses

ZHANG Ruiteng1,2,FU Qiushi1,ZHANG Lei3,LI Shuangtao3,GUO Yangdong3,ZHANG Hong4, LI Lingzhi2,LI Haiping2,*,and WANG Huaisong1,*   

  1. 1Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;2College of
    Horticulture,Shanxi Agricultural University,Taigu,Shanxi 030801,China;3College of Horticulture,China Agricultural
    University,Beijing 100193,China;4Hami Melon Research Center,Xinjiang Academy of Agricultural Sciences,Urumqi
    830091,China
  • Online:2017-07-25 Published:2017-07-25

摘要:

为了探究非生物胁迫下甜瓜N–乙酰氨基葡萄糖基转移酶基因(CmGnT)的作用机制,根
据甜瓜cDNA 序列设计引物,利用RT-PCR 技术克隆得到CmGnT。生物信息学分析表明,CmGnT 的cDNA
全长为2 193 bp,开放阅读框(ORF)为1 179 bp,编码392 个氨基酸。CmGnT 蛋白分子量约为46 kD,
理论等电点(pI)为7.93。蛋白结构分析表明,CmGnT 含有β–1,4–N–乙酰氨基葡萄糖转移酶结构域,
属于糖基转移酶17 家族。该基因编码的蛋白有1 个强的跨膜螺旋结构。进化树分析表明,CmGnT 氨基
酸序列与黄瓜CsGnT(登录号:XP_004135275.1)亲缘关系最近,同源性为99.74%,与西瓜CsGnT 的同
源性为97.70%。实时荧光定量PCR 分析结果表明,在NaCl、PEG、H2O2 和ABA 等非生物胁迫下的甜瓜
叶片中 CmGnT 都显著上调表达,表明CmGnT 受非生物胁迫诱导,推测其在甜瓜响09 应非生物胁迫过
程中起到重要作用。

关键词: 甜瓜, N&ndash, 乙酰氨基葡萄糖基转移酶, 基因克隆, 基因表达, 非生物胁迫

Abstract:

The aim of this study was to explore the mechanisms of N-acetylglucosaminyltransferase
gene(CmGnT)in melon under abiotic stresses. Primers were designed according to cDNA sequence of
melon,and CmGnT was cloned by using RT-PCR technique. The full-length cDNA of CmGnT was 2 193
bp,and the open reading frame(ORF)was 1 179 bp,encoding 392 amino acids. The molecular weight of
CmGnT protein was about 46 kD,and the theoretical isoelectric point(pI)was 7.93. Protein structure analysis showed that CmGnT contained the β-1,4-N-acetylglucosaminyltransferase domain , which
belonged to the glycosyltransferases 17 family. The protein had 1 strong transmembrane helices.
Phylogenetic analysis showed that CmGnT and CsGnT(accession number:XP_004135275.1)had the
closest genetic relationship,and the homology was 99.74%. Homology with watermelon was 97.70%.
qRT-PCR analysis showed that the expressions of CmGnT in leaf were significantly up-regulated under
NaCl,PEG,H2O2 and ABA stresses,which indicated that CmGnT was induced by abiotic stresses. The
results suggested that this gene in melon played an important role in response to abiotic stresses.

Key words: melo, N-acetylglucosaminyltransferase, gene clone, gene expression, abiotic stresses