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园艺学报 ›› 2017, Vol. 44 ›› Issue (7): 1355-1362.doi: 10.16420/j.issn.0513-353x.2017-0047

• 研究报告 • 上一篇    下一篇

苹果抗炭疽菌叶枯病基因相关的4 个分子标记的准确性验证

刘春晓1,兰进好2,侯鸿敏1,张玉刚1,戴洪义1,刘源霞1,*   

  1. 1 青岛农业大学园艺学院,青岛市园艺植物遗传改良与育种重点实验室,山东青岛 266109;2 青岛农业大学农学
    与植物保护学院,山东青岛 266109
  • 出版日期:2017-07-25 发布日期:2017-07-25

Accuracy Test of Four Molecular Markers of Glomerella Leaf Spot Resistant Gene in Apple Cultivars

LIU Chunxiao1,LAN Jinhao2,HOU Hongmin1,ZHANG Yugang1,DAI Hongyi1,and LIU Yuanxia1,*   

  1. 1Qingdao Key Laboratory of Genetic Improvement and Breeding in Horticultural Plants,College of Horticulture,
    Qingdao Agricultural University,Qingdao,Shandong 266109,China; 2 College of Agronomy and Crop Protection,Qingdao
    Agricultural University,Qingdao,Shandong 266109,China
  • Online:2017-07-25 Published:2017-07-25

摘要:

利用50 个苹果栽培品种(系)对与抗炭疽菌叶枯病基因(Rgls)位点紧密连锁的4 个分子标
记S0405127(SSR)、S0304673(SSR)、SNP4236 和InDel4254 的准确性进行验证。室内离体接种表型鉴
定及分子标记基因型鉴定的结果表明,4 个分子标记验证苹果炭疽菌叶枯病抗性的准确率分别为90%、
94%、96%和92%。

关键词: 苹果, 炭疽菌叶枯病, 分子标记, 可靠性验证

Abstract:

In the present study,the accuracy of the four molecular markers tightly linked with Rgls
locus,S0405127(SSR),S0304673(SSR),SNP4236,and InDel4254 were tested based on the resistant
phenotypic and genotypic identification of 50 apple cultivars. The results indicated that the accuracy of the
four molecular markers were 90%,94%,96%,92%,respectively. The study could provide breeders an
effective method to speed up breeding program by using these molecular markers for the pre-selection of
the hybrid seedlings resistant to the Glomerella leaf spot in apple.

Key words: apple, Glomerella leaf spot, molecular marker, accuracy test