关键词: 桃；果实；叶片；土壤；草甘膦；氨甲基膦酸；高效液相色谱&mdash, 串联质谱

Abstract:

An analytical method for the determination of glyphosate（PMG）and aminomethyl- phosphonic acid（AMPA）in peach fruit，leaf and soil was established using high performance liquid chromatography-tandem mass spectrometry（HPLC-MS/MS）. The samples were extracted with NaHCO₃ water solution and dichloromethane，derived with 9-fluorenylmethyl chloroformate（FMOC-Cl）at room temperature for 3 h，and analyzed in multiple reaction monitoring（MRM）mode. The results indicated good linearity with correlation coefficients greater than 0.999 in the concentration range of 1–2 000 μg · L^-1. The average recoveries of PMG and AMPA in each matrixes were found between 76.7% and 93.0% at 0.01–1.0 mg · kg^-1，with relative standard deviations（RSD）of 2.2%–8.7%. The limits of  quantification（LOQ）were 0.01 mg · kg^-1. The method was suitable for rapid determination of PMG and AMPA residues in fruit and leaf of peach and soil.

Key words: peach, fruit, leaf, soil, glyphosate, aminonethylphosphonic acid, HPLC-MS/MS