https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2017, Vol. 44 ›› Issue (3): 504-514.doi: 10.16420/j.issn.0513-353x.2016-0593

• 研究论文 • 上一篇    下一篇

几种植物花分生组织决定基因APETALA1密码子使用模式比较

吴彦庆1,2,李志远1,2,赵大球2,陶 俊2,*   

  1. 1扬州大学动物科学与技术学院,江苏扬州 225009;2扬州大学园艺与植物保护学院,江苏省作物遗传生理重点实验室,江苏扬州 225009
  • 出版日期:2017-03-25 发布日期:2017-03-25

Comparative Analysis of Codon Usage Patterns in APETALA1 Gene Across Several Plant Species

WU Yanqing1,2,LI Zhiyuan1,2,ZHAO Daqiu2,and TAO Jun2,*   

  1. 1College of Animal Science and TechnologyYangzhou UniversityYangzhouJiangsu 225009China2Key Laboratory of Crop Genetics and PhysiologyCollege of Horticulture and Plant ProtectionYangzhou UniversityYangzhouJiangsu 225009China
  • Online:2017-03-25 Published:2017-03-25

摘要:

为了揭示花分生组织决定基因AP1APETALA1)的分子机制以及物种间的进化关系,从GenBank数据库挑选8个不同植物AP1基因编码序列,利用R软件分别计算GC含量、有效密码子数(Effective number of codons,ENC)、同义密码子相对使用度(Relative synonymous codon usage,RSCU)、相对密码子使用偏好性(Relative codon usage bias,RCBS)等参数,分析比较不同植物AP1基因密码子使用模式中密码子偏好性和碱基组成动力学。结果表明,不同植物AP1基因密码子使用受到GC偏好性(GC bias)影响,特别是GC3s;大多数最优密码子偏好使用以A/U结尾,其中不同植物AP1基因中ACA、AUA和CAG均表现为较高的RSCU值,为最优势密码子;芍药(Paeonia lactiflora)和牡丹(Paeonia suffruticosaAP1基因的密码子使用特点相似,碧桃(Prunus persica)和苹果(Malus × domestica)相似;较低的最优密码子频率Fop值和较高的ENC值暗示了不同植物AP1基因在密码子使用中具有一个较弱的偏好性。

关键词: APETALA1, 植物, 密码子使用偏好性, 碱基组成动力学

Abstract:

To reveal the molecular mechanism of APETALA1AP1)gene and the evolution among different plants,we selected the complete nucleotide coding sequences of AP1 gene from 8 different plant species for CUB analysis. Using R software,we performed a comparative analysis of codon bias and compositional dynamics of codon usage patterns in AP1 gene across eight different plant species by determining the GC contents,several genetic indices namely effective number of codons(ENC),relative synonymous codon usage(RSCU),and relative codon usage bias(RCBS)etc. Results showed codon usage in AP1 gene in plants were influenced by GC bias,mainly due to GC3s. The majority of optimal codons were A/U ending for the corresponding amino acid,of which ACA,AUA and CAG were over-represented codons in the AP1 gene of all the selected plant species. The codon usage pattern for AP1 in Paeonia lactiflora showed resemblance to that of Paeonia suffruticosa;similarly,and Prunus persica to Malus × domestica. Low Fop(Frequency of optimal codon)and high ENC values suggested a weaker codon bias observed for the AP1 gene in different plant species.

Key words: APETALA1, plant, codon usage bias, compositional dynamics