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园艺学报 ›› 2017, Vol. 44 ›› Issue (3): 422-430.doi: 10.16420/j.issn.0513-353x.2016-0548

• 研究论文 • 上一篇    下一篇

李果实中多酚含量及其抗氧化能力研究

周丹蓉,方智振,叶新福*,潘少霖,姜翠翠   

  1. 福建省农业科学院果树研究所,福州 350013
  • 出版日期:2017-03-25 发布日期:2017-03-25
  • 基金资助:

    福建省重大专项(2013NZ0002-1);农业部热带作物种质资源保护项目(16RZZY-12);福建省自然科学基金项目(2016J01123,2015J05058);福建省公益类科研院所基本科研专项(2015R1014-9);福建省农业科学院青年英才项目(YC2015-9)

Concentration and Antioxidant Capacity of Polyphenol from Plum Cultivars

ZHOU Danrong,FANG Zhizhen,YE Xinfu*,PAN Shaolin,and JIANG Cuicui   

  1. Fruit Research InstituteFujian Academy of Agricultural SciencesFuzhou 350013China
  • Online:2017-03-25 Published:2017-03-25

摘要:

为分析不同李品种果实中多酚含量及其抗氧化能力,以14个李品种为试材,测定果皮和果肉中的多酚含量,采用超氧自由基( )清除法、羟自由基(OH·)清除法、DPPH法、FRAP法和TBARS法测定抗氧化能力,比较多酚含量、抗氧化能力的差异,分析多酚含量与抗氧化能力的相关性,比较抗氧化能力测定方法间的相关性。结果表明:(1)李果实中富含多酚并且品种间存在差异,不同品种果皮中含量为1.40 ~ 3.37 mg · g-1 FW,果肉中含量为0.50 ~ 0.97 mg · g-1 FW,果皮中含量远大于果肉中含量;果皮中多酚含量较高的李品种为皇后李、猪肝李,其次为黑宝石李、红玫瑰李;果肉中多酚含量最高的为秋姬李,其次为威克逊李。(2)李果皮、果肉多酚提取物均具有较强的抗氧化能力,果皮提取物综合抗氧化能力较强的为皇后李、黑宝石李和猪肝李,果肉的为红美丽李、红玫瑰李和秋姬李。(3)果皮中多酚含量与清除   、OH·能力及DPPH、FRAP、TBARS均呈极显著正相关,果肉中多酚含量与抗氧化能力呈显著正相关。(4)对5种方法的相关性分析表明,各方法从不同的角度评价李果多酚抗氧化能力,方法间均具有很好的相关性,DPPH法与FRAP法综合评价最佳,可作为主要选择。

关键词: 李, 果皮, 果肉, 多酚, 抗氧化

Abstract:

Phenolic content and antioxidant capacity of fruit extracts from 14 plum cultivars,including peel and flesh,were assayed. Polyphenol contents in peel of plums ranged from 1.40 to 3.37 mg · g-1 FW,which was expressed as gallic acid equivalents,for fresh weight;polyphenol contents in flesh ranged from 0.50 to 0.97 mg · g-1 FW. The polyphenol contents,both in peel and flesh,were significant different among cultivars(P < 0.01). However,the contents were great higher in peel than that in flesh. The contents of polyphenol in peel were high in Huanghouli and Zhuganli,and followed by Heibaoshili and Hongmeiguili;and the content in flesh was the highest in Qiujili,and followed by Weikexunli. The scavenging activities of superoxide anion radical(   ),hydroxyl radical(OH·),DPPH,FRAP and TBARS were employed to evaluate the antioxidant activity of plums. The first three main antioxidant cultivars of peel were Huanghouli,Heibaoshili and Zhuganli. And that of flesh was Hongmeilili,Hongmeiguili and Qiujili. It showed that there were remarkable correlation between polyphenol concentrations and antioxidant capacity. According to the relations and comprehensive evaluation,DPPH and FRAP were considered to be the excellent method which used to evaluate antioxidant capacity of plum. There,polyphenol from plums may provide practical antioxidant,which can reduce the harm of free radicals and help people to keep fit.

Key words: Prunus salicina, peel, flesh, polyphenol, antioxidant capacity