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园艺学报 ›› 2007, Vol. 34 ›› Issue (4): 929-934.

• 蔬菜 • 上一篇    下一篇

SRAP在葱栽培品种遗传多样性研究中的适用性分析

李慧芝; 尹燕枰*; 张春庆;张 敏; 李建敏   

  1. (山东农业大学农学院, 作物生物学国家重点实验室, 山东泰安271018)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-08-25 发布日期:2007-08-25

Evaluation of Application of SRAP on Analysis of Genetic Diversity in Cultivars of Allium fistulosum L.

LI Hui-zhi; YIN Yan-p ing*; ZHANG Chun-qing;ZHANG Min;LI Jian-min
  

  1. (Agronomy College, Shandong Agricultural University, State Key Laboratory of Crop Biology, Tai'an, Shandong 271018,China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-08-25 Published:2007-08-25

摘要: 为评价SRAP技术对葱品种进行鉴定和遗传关系分析的适用性, 对20个葱栽培品种的表型特
征进行了观察记载, 利用256个SRAP引物组合对其进行了遗传多样性研究。结果表明: (1) 256个SRAP
引物组合中有161个引物组合产生多态性条带, 占所用引物组合数的62.9%。161个引物组合共产生336
条多态性条带, 不同引物组合产生的多态性条带数为1~6个, 平均2.1个。20个葱栽培品种遗传相似系
数变幅为0.464~0.938, 平均0.703。(2) 依据SRAP进行聚类分析的分类结果与依据表型特征分类的结
果一致。上述结果说明SRAP标记可以在葱栽培品种的鉴定和遗传多样性研究中应用。

关键词: 葱, 栽培品种, SRAP, 遗传多样性, 适用性

Abstract: In order to evaluate the app lication of SRAP in identification and genetic relationship analysis
on cultivars ofA llium f istulosum L. , 20 cultivarswere used in this experiment. We investigated the morphological characters and analyzed the genetic diversity of 20 cultivars with SRAP markers. 256 p rimer pairs were
tested, 161 (62.9% ) of which generated polymorphic bands. A total of 336 polymorphic bands were observed. The number of polymorphic bands detected by an individual p rimer pair ranged from one to six, with
an average number of 2.1. Genetic similarity (GS) coefficients among 20 cultivars based on the SRAP data
ranged from 0.464 to 0.938, with a mean GS coefficient of 0.703. A dendrogram was generated using cluster
analysis, which revealed phylogenetic relationship s among 20 cultivars. The result was in agreement with the
common classification based on morphologic traits, which suggested that SRAP marker could be reliably used
in cultivar identification and genetic diversity analysis of A. fistulosum L.

Key words: Allium fistulosum L., Cultivar, SRAP, Genetic diversity, Application