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园艺学报 ›› 2017, Vol. 44 ›› Issue (2): 233-244.doi: 10.16420/j.issn.0513-353x.2016-0388

• 研究论文 • 上一篇    下一篇

Aux/IAA家族基因鉴定及在果实成熟过程中的表达分析

曾文芳,王小贝,潘  磊,牛  良,鲁振华,崔国朝,王志强*   

  1. 中国农业科学院郑州果树研究所,国家桃葡萄改良中心,农业部果树育种技术重点实验室,郑州450009
  • 出版日期:2017-02-25 发布日期:2017-02-25
  • 基金资助:

    河南省科技厅基础前沿项目(152300410137);中国农业科学院科技创新工程项目(CAAS-ASTIP-2017-ZFRI);国家自然科学基金项目(31501732)

Identification and Expression Profiling of Aux/IAA Family Gene During Peach Fruit Ripening

ZENG Wenfang,WANG Xiaobei,PAN Lei,NIU Liang,LU Zhenhua,CUI Guochao,and WANG Zhiqiang*   

  1. Zhengzhou Fruit Research InstituteChinese Academy of Agricultural SciencesNational Peach and Grape Improvement CenterKey Laboratory of Fruit Breeding Technology of Ministry of AgricultureZhengzhou 450009China
  • Online:2017-02-25 Published:2017-02-25

摘要:

通过生物信息学鉴定桃基因组中生长素/吲哚乙酸(Aux/IAA)基因家族,并对其在溶质型和硬质型桃果实成熟阶段的表达水平进行qRT-PCR检测。结果表明:桃Aux/IAA基因家族包含22个成员,这些基因集中分布在5条染色体上,以第1条染色体上含有最多,为8个。大部分Aux/IAA基因包含高度保守的Ⅰ、Ⅱ、Ⅲ和Ⅳ功能域,聚类分析表明其分为10类。基因结构分析显示这些基因包含2 ~ 5个外显子。荧光定量PCR分析表明10个Aux/IAA基因在溶质型桃果实的表达量高于对应时期硬质型桃果实,其中5个基因随着溶质型桃果实的成熟上调表达,特别是ppa010303mppa010871mppa020369m。试验结果表明桃Aux/IAA家族成员在结构上高度保守,其中多个成员(尤其是ppa010303mppa010871mppa020369m等)可能参与调控桃果实的成熟。

关键词: 桃, 生长素/吲哚乙酸基因, 果实成熟, 生长素

Abstract:

Auxin/indole-3-acetic acid(Aux/IAA)is involved in plant growth,development,and multiple other processes. In this study,the Aux/IAA gene family in the peach genome was identified and analyzed by bioinformatics,additionally,the expression levels of Aux/IAA genes were detected by qRT-PCR in melting-flesh and stony-hard peach fruits during fruit ripening. The results showed that 22 PpAux/IAA candidate genes were found in the peach genome. A chromosome mapping analysis revealed that PpAux/IAA genes were distributed with different densities over 5 chromosomes,with the largest number of PpAux/IAA genes on chromosome 1(8 genes). The DNA-binding and Aux/IAA domains,as well as motif Ⅰ,Ⅱ,Ⅲ and Ⅳ of the PpAux/IAA gene family were highly conserved. The phylogenetic analysis revealed that PpAux/IAA gene family was divided into 10 groups. The results of an intron-exon structure analysis indicated that PpAux/IAA gene family members were composed of 2–5 exons. qRT-PCR results indicated that the expression level of ten PpAux/IAA genes in melting-flesh peach fruits were higher than in stony-hard peach fruits,and the expression level of 5 genes were upregulated in melting-flesh fruits during fruit ripening,especially for ppa010303mppa010871m,and ppa020369m. These results suggested that the PpAux/IAA gene family members are highly and structurally conserved,and some of them(especially for ppa010303mppa010871m and ppa020369m)are involved in regulating peach fruit ripening.

Key words: peach, Prunus persica, Aux/IAA gene family, fruit ripening, indole-3-acetic-acid

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