关键词: 小花草玉梅, 花器官, 变异, 分子鉴定, AP3-3基因

Abstract:

After several years’ survey，a serious of nature floral organs variants of Anemone rivularis var. flore-minore were determined. Based on varation organ and varation level，variants were divided into 5 types：white，green-white，five-flap，green and extreme variation. The variation mechanism was studied preliminarily through molecular identification，morphological observations，and AP3-3 genes analysis. The results indicate that both ITS2 and rbcL sequences from normal and variants had no difference after comparison，so the normal and variant plants were identified as the same species. Variant flowers had big differences in morphologys，which existed a variation gradient from outside，and proportion and number of floral organs were all unstable except tepals numbers from normal and five-flap flowers，besides，tepals proportion was negative correlation with stamens/carpels，so it indicated that increased tepals in variant flowers were very likely transformed from stamens and carpels. Based on AP3-3 genes from normal and green-white plants，the rest AP3-3 genes were amplified. Sequence alignment of AP3-3 from normal and variant plants showed that regulation region had a insertion and CDS region had 1 base deletion. Both of insertion and deletion might involve in variation.

Key words: Anemone rivularis var. flore-minore, floral organ, variant, molecular identification, AP3-3 gene