关键词: 香蕉, TIFY, 转录因子, 果实成熟, 基因表达

Abstract:

TIFY transcription factors（TFs）play an essential role in plant growth and development as well as environmental adaptation. However，the involvement of TIFY TFs in fruit ripening remains largely unknown. In this work，a gene encoding TIFY subgroup member，which was designated as MaTIFY1，was isolated from banana fruit. MaTIFY1 contains an Open Reading Frame（ORF）of 681 bp，encoding a peptide of 227 aa，with molecular weight of 24.97 kD and pI of 7.85. Subcellular localization and transactivation activity assays indicated that MaTIFY1 was targeted to nucleus and owned transcriptional activation activity. qRT-PCR analysis revealed that expression of MaTIFY1 was gradually increased along with the progression of fruit ripening，and was induced by propylene. Moreover，the promoter activity of MaTIFY1 was also activated by ethylene treatment，further supporting the role of MaTIFY1 in fruit ripening. In addition，the recombinant MaTIFY1 protein was available，which provided the basis for further investigation of MaTIFY1’s functions.

Key words: banana, TIFY, transcription factor, fruit ripening, gene expression