关键词: 苹果, G 蛋白偶联受体, MdGCR1, 抗旱胁迫

Abstract:

The G protein-coupled receptor gene MdGCR1 was cloned by combination of the
homologous clone and PCR technology from apple（Malus × domestica‘Royal Gala’）. The Open Reading
Frame（ORF）of MdGCR1 contained 960 bp，encoding a protein of 319 amino acid residues. Phylogenetic
analysis showed that MdGCR1 had the highest evolutionary relationship with GCR1 from pear. The results
of transcriptional level showed that expression of MdGCR1 was main in root，stem and leaf and the
expression in flower and fruit tissues was lower. The gene expression of MdGCR1 was induced by multiple
stress treatments and was regulated in response to hormones. Transgenic tobacco was generated by
Agrobacterium-mediated genetic transformation. Tobacco that ectopic expression of MdGCR1 showed
more sensibility to drought stress，indicating that G protein-coupled receptor gene MdGCR1 played an
important role in plant drought stress.