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园艺学报 ›› 2016, Vol. 43 ›› Issue (8): 1593-1604.doi: 10.16420/j.issn.0513-353x.2016-0094

• 研究报告 • 上一篇    下一篇

板栗雌雄花发育相关的MADS-box基因发掘与表达分析

邱文明,徐育海*,何秀娟   

  1. (湖北省农业科学院果树茶叶研究所,湖北省农业科技创新中心果树茶叶研究分中心,武汉 430064)
  • 出版日期:2016-08-25 发布日期:2016-08-25
  • 基金资助:

    湖北省农业科技创新中心资助项目(2011-620-005-003-02);湖北省农业科学院青年科学基金项目(2014NKYJJ08);湖北省农业科学院果树茶叶研究所青年科学基金项目(GCJJ201401)

Identification and Expression Analysis of MADS-box Genes Related to Female and Male Flowers Development in Chestnut

QIU Wen-ming,XU Yu-hai*,and HE Xiu-juan   

  1. Institute of Fruit and TeaHubei Academy of Agricultural SciencesFruit and Tea Subcenter of Hubei Innovation Center of Agricultural Science and TechnologyWuhan 430064China
  • Online:2016-08-25 Published:2016-08-25

摘要:

为筛选板栗混合花序中雌雄花形成与发育关键基因,基于板栗雌雄花表达谱测序结果,从差异表达基因中分离到17个MADS-box基因。通过序列结构分析,发现其中2个基因缺少半保守的K盒,为Ⅰ型MADS-box基因,另外15个基因为Ⅱ型MADS-box基因。系统进化关系分析显示,13个包含完整读码框的MADS-box基因中有2个B类基因,1个C/D类基因和3个E类基因。荧光定量PCR结果证实这些MADS-box基因在板栗早期混合花序中的雌雄花之间表达差异显著(变化倍数 > 2),且部分B、C/D和E类基因在板栗幼叶、纯雄花和雌花中的表达变化趋势相似,推测这些MADS-box基因在板栗雌雄花形成和发育中起重要调控作用

关键词: 板栗, 混合花序, MADS-box基因

Abstract:

To screen key genes involved in the differentiation and development of flowers in chestnut (Castanea mollissima Blume),17 MADS-box genes were identified from the differentially expressed genes(DEGs)between female and male flowers of the mixed inflorescence by RNA-seq analysis. Gene structure analysis showed that two genes were Ⅰ-type MADS box genes as lack of semi-conserved K motif,while the other fifteen genes belonged to Ⅱ-type MADS-box group. And phylogenetic analysis demonstrated that among the 13 MADS-box genes with complete open reading frames(ORFs),there were two B-class genes,one C/D-class gene and three E-class genes based on the ABCDE model hypothesis. Furthermore,quantitative real time PCR was used to investigate the expression profiling of these genes in young leaves,male inflorescences at early stage,male and female flowers in the mixed inflorescence. And for one thing the results confirmed that these genes were DEGs(fold change > 2),for another it indicated that some of these MADS-box genes showed very similar expression pattern. And it assumed that these MADS-box genes might play important roles in the differentiation and development of flowers in chestnut.

Key words: chestnut, mixed inflorescence, MADS-box genes

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