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园艺学报 ›› 2016, Vol. 43 ›› Issue (8): 1577-1584.doi: 10.16420/j.issn.0513-353x.2016-0078

• 研究报告 • 上一篇    下一篇

Ty1-copia类反转录转座子GAG开放阅读框预测和光响应下的表达分析

蒋  爽1,2,骆  军2,孙永旺1,蔡丹英3,滕元文1,*   

  1. 1浙江大学园艺系,农业部园艺植物生长发育与品质调控重点开放实验室,杭州 310058;2上海市农业科学院林木果树研究所,上海 201403;3浙江省农业科学院园艺研究所,杭州 310021)
  • 出版日期:2016-08-25 发布日期:2016-08-25
  • 基金资助:

    浙江省重点科技创新团队项目(2013TD05)

Prediction of ORF for GAG of Ty1-copia Retrotransposons in Pear and Its Expression Analysis in Response to Light

JIANG Shuang1,2,LUO Jun2,SUN Yong-wang1,CAI Dan-ying3,and TENG Yuan-wen1,*   

  1. 1The State Agricultural Ministry Laboratory of Horticultural Plant GrowthDevelopment & Quality ImprovementDepartment of HorticultureZhejiang UniversityHangzhou 310058China2Forestry and Fruit Tree Research InstituteShanghai Academy of Agriculture SciencesShanghai 201403China3Institute of HorticultureZhejiang Academy of Agricultural SciencesHangzhou 310021China
  • Online:2016-08-25 Published:2016-08-25

摘要:

基于生物信息学方法,对‘砀山酥梨’基因组中Ty1-copia类型的GAG开放阅读框进行预测,共获得315条GAG序列。通过系统聚类,梨基因组中GAG序列可分为7个支系,第一和第三支系成员较多且保守性高。序列比对结果显示,不同支系GAG序列具有较高的异质性。在‘美人酥’梨的套袋和摘袋后的果皮中发现了34个gag成员,其中有6个(Ppgag16Ppgag19Ppgag23Ppgag27Ppgag29Ppgag30)为高丰度表达成员。遮光条件下,‘美人酥’果皮中有部分gag成员发生表达,说明copia类反转座子不仅仅受光照影响发生表达。在摘袋后,‘美人酥’果皮中有2个成员(Ppgag5Ppgag23)发生明显上调表达,说明梨中部分反转录转座子的转录受到光照的调控。

关键词: 梨, Ty1-copia, GAG, 表达分析

Abstract:

Different types of sequences of ORF for GAG in the whole genome of‘Dangshan Suli’(Pyrus pyrifolia White Pear Group)were predicted by bioinformatics methods. A total of 315 GAGsequences were obtained from Ty1-copia retrotransposons. The cluster analysis indicated that there were seven groups,among which,groups one and three had many conserved members. Sequence alignment of all GAG sequences in pear genome showed a high heterogeneity. A total of 34 members were expressed in bagging and bag-removed‘Meirensu’pear fruit,of which,six members(Ppgag16Ppgag19Ppgag23Ppgag27Ppgag29,and Ppgag30)were highly expressed. In bagging-treated fruit,some members of gag were expressed,suggesting that lightwas not a sole factor to activatethe expression of copia retrotransposons. In bag-removed fruit,the expression of two members(Ppgag5 and Ppgag23)were up-regulated,indicating that some members of the retrotransposons were regulated by light.

Key words: Pyrus, Ty1-copia, GAG, expression analysis

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