https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
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https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
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https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2016, Vol. 43 ›› Issue (8): 1429-1436.doi: 10.16420/j.issn.0513-353x.2016-0242

• 果树 •    下一篇

苹果细胞分裂素O–糖基转移酶基因MdZOG1的分离与功能鉴定

安建平1,*,宋来庆2,*,赵玲玲2,由春香1,王小非1,**,郝玉金1,**   

  1. 1山东农业大学园艺科学与工程学院,作物生物学国家重点实验室,山东泰安 271018;2烟台市农业科学研究院,山东烟台265599
  • 出版日期:2016-08-25 发布日期:2016-08-25
  • 基金资助:

    国家自然科学基金项目(31430074);山东省农业良种工程和产业体系岗位专家项目(SDAIT-03-022-03)

Molecular Cloning and Functional Characterization of a Cytokinin O-glycosyltransferase Gene MdZOG1 in Apple

AN Jian-ping1,*,SONG Lai-qing2,*,ZHAO Ling-ling2,YOU Chun-xiang1,WANG Xiao-fei1,**,and HAO Yu-jin1,**   

  1. 1College of Horticulture Science and EngineeringShandong Agricultural UniversityState Key Laboratory of Crop BiologyTai’anShandong 271018China2Yantai Academy of Agricultural SciencesYantaiShandong 265599China
  • Online:2016-08-25 Published:2016-08-25

摘要:

以‘嘎拉’苹果为材料,采用同源克隆和PCR技术分离了苹果细胞分裂素O–糖基转移酶基因MdZOG1MdZOG1的开放阅读框(ORF)长度为762 bp,编码含有253个氨基酸的蛋白。系统进化树分析显示,MdZOG1与PbZOG1亲缘关系最近。基因表达分析显示MdZOG1主要在苹果根和茎中表达,在花和果实中的表达量较低。通过农杆菌介导的遗传转化获得转MdZOG1拟南芥和烟草。干旱处理试验结果显示:超量表达MdZOG1显著提高拟南芥和烟草植株的抗旱能力,表明苹果细胞分裂素O–糖基转移酶在植物抗旱胁迫中发挥重要作用。

关键词: 苹果, 细胞分裂素O–糖基转移酶, MdZOG1, 抗旱胁迫

Abstract:

The cytokinin O-glycosyltransferase gene MdZOG1 was cloned by combination of the homologous clone and PCR technology from apple(Malus × domestica‘Gala’). The Open Reading Frame (ORF)of MdZOG1 contained 762 bp,encoding a protein of 253 amino acid residues. Phylogenetic analysis showed that MdZOG1 had the highest evolutionary relationship with PbZOG1. The transcriptional analysis results showed that expression of MdZOG1 was mainly in root and stem,the expression of MdZOG1 in flower and fruit tissues was lower than other tissues. Transgenic Arabidopsis and tobacco were generated by Agrobacterium-mediated genetic transformation. Ectopic expression MdZOG1 in Arabidopsis and tobacco significantly enhanced resistance to water stress,indicating that cytokinin O-glycosyltransferase played an important role in plant drought stress.

Key words: apple, cytokinin O-glycosyltransferase, MdZOG1, drought stress

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