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园艺学报 ›› 2016, Vol. 43 ›› Issue (5): 991-997.doi: 10.16420/j.issn.0513-353x.2016-0009

• 研究报告 • 上一篇    下一篇

西瓜花叶病毒新疆鄯善分离物的纯化及不同甜瓜品种的抗病性鉴定

王 东,杜姗姗,李 冠*   

  1. (新疆大学生命科学与技术学院,乌鲁木齐 830046)
  • 出版日期:2016-05-25 发布日期:2016-05-25

Purification of a Watermelon mosaic virus Isolate from Shanshan County and Evaluation for the Virus Resistance of Various Melon Cultivars

WANG Dong,DU Shan-shan,and LI Guan*   

  1. (College of Life Science and Technology,Xinjiang University,Urumqi 830046,China)
  • Online:2016-05-25 Published:2016-05-25

摘要:

从新疆哈密瓜(Cucumis melo L.)主产区之一的鄯善县采集表现病毒病症状的哈密瓜嫩叶,经昆诺藜连续3次单斑分离纯化后,采用RT-PCR扩增、克隆和测序分析,将该分离物鉴定为西瓜花叶病毒(Watermelon mosaic virusWMV)。利用该分离物采用汁液摩擦接种法对12个常见的甜瓜商业品种和13个国际通用的甜瓜白粉病生理小种鉴别寄主进行室内抗病性鉴定,结果表明,所有供试品种中没有免疫和高抗品种表现抗病、中抗、感病和高感的品种分别有111103个,其中表现抗病的为Iran H

关键词: 甜瓜, 西瓜花叶病毒, 抗性评价, 分离物

Abstract:

As one of the most important economic crops in XinjiangHami melonCucumis melo L.is of high reputation over the world. Howevervirus diseases are currently a serious threat for sustainable production of Hami melon because of the lack of effective antivirus chemical and appropriate virus-control method. To develop virus-resistant varietiesyoung leaves of Hami melon with virus-infected symptoms were collected from Shanshan countywhich is one of the major Hami melon growing locations in Xinjiang. This specimen was biologically cloned by three local lesion passages in Chenopodium quinoa. Such biologically cloned virus is hereafter called an isolatewhich was further identified by RT-PCR amplificationmolecular cloning and sequencing. The results showed that the purified isolate was Watermelon mosaic virusWMV),which was then inoculated to some common commercial melon varieties and 13 international universal hosts for identification of melon powdery mildew physiological races by mechanical rubbingthe laboratory evaluation of virus resistance indicated that there was no immune and high resistance varieties in the present study. The number of resistancemoderate resistancesensitivityand high sensitivity varieties were 11110and 3respectivelyout of thesecultivar Iran H showed resistance to WMV isolate.

Key words: melon, Watermelon mosaic virus, evaluation for the resistance, isolate

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