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园艺学报 ›› 2007, Vol. 34 ›› Issue (4): 841-846.

• 果树 • 上一篇    下一篇

离体梨花柱S2RNa se对自体花粉管超微结构变化的影响

刘珠琴1;张绍铃1,*;徐国华2
  

  1. (1 南京农业大学园艺学院, 南京210095; 2 江苏教育学院生物系, 南京210013)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-08-25 发布日期:2007-08-25

Effect of Pear Stylar S-RNa se on Self-pollen Tube Ultrastructure in Vitro

LIU Zhu-qin1;ZHANG Shao-ling1,*;XU Guo-hua2
  

  1. (1College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China; 2Department of Biology, Jiangsu Institute ofEducation, Nanjing 210013, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-08-25 Published:2007-08-25

摘要: 采用透射电子显微镜研究了离体条件下‘今村秋’、‘丰水’梨花柱S2RNase对‘今村秋’花
粉管生长过程中花粉管超微结构的影响。‘丰水’花柱S2RNase处理的‘今村秋’花粉管(异体, 亲和)
和对照花粉管结构在生长过程中表现正常, 即花粉管顶端生长区域充满细胞质和细胞器, 结构完整, 花粉
管壁上没有胼胝质层分布。而‘今村秋’花柱S-RNase处理的‘今村秋’花粉管(自体, 不亲和) 超微结
构随时间的延长出现衰退现象。处理3 h时, 自体花粉管内充满细胞质和细胞器, 结构完整, 但在花粉管
的极顶端分泌小泡融合, 并且此区域的细胞壁比后部的薄; 处理8 h后, 细胞器出现一定程度的衰退现象,
线粒体膨大, 嵴减少或消失, 内质网膨大并包围在液泡和其它细胞器周围, 液泡融合并侵蚀周围的细胞质
和细胞器; 到24 h时, 自体花粉管内线粒体、内质网等细胞器消失, 只有靠近花粉管前端有少量细胞质存
在, 细胞壁增厚, 并且有一层厚厚的胼胝质层紧靠细胞壁。结果表明, 花柱S2RNase能引起自体花粉管的
衰退, 从而抑制自体花粉管生长。

关键词: 梨, 自交不亲和性, S2RNase, 花粉管, 超微结构

Abstract: The effects of pear stylar S-RNase on compatible and incompatible pollen tube ultrastructure
in vitro were studied with transmission electron microscope. Ultrastructure of compatible pollen tube and con
trolwas natural during growth, i.e. cytop lasm and organelles filled tube growth tip and their configurations
were intact. There was no callose along the cell wall. While ultrastructure of incompatible tube degenerated
gradually followed by treatment time. After treated for 3 h incompatible tube was full of cytop lasm and organ
elles. There was no callose along the cell wall. But secreting vacuoles fused at the apex of tube and the cell
wall was thinner than that of others. Eight hours after treatment mitochondria appeared swelling, cristae de
creased or disappeared, ER dilated and wrapped around vacuoles and other organelles, vacuoles fused and
swallowed cytop lasm and organelles. After 24 h, mitochondria and ER disappeared, only a little of cytop lasm
laid near the tube tip and the shank nearlywere emp ty. Cellwalls became thicker and a layer of callose laid a
long the cell walls. The result hits pear stylar S2RNase could cause degeneration of incompatible pollen tube
and inhibited incompatible tube growth.

Key words: Pear, Self-incompatibility, S-RNase, Pollen tube, Ultrastructure