https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2016, Vol. 43 ›› Issue (5): 817-828.doi: 10.16420/j.issn.0513-353x.2015-0873

• 果树 •    下一篇

5-ALA诱导的黄酮醇积累参与调节苹果叶片气孔开度

刘龙博,安玉艳,熊丽君,汪良驹*   

  1. (南京农业大学园艺学院,南京 210095)
  • 出版日期:2016-05-25 发布日期:2016-05-25

Flavonols Induced by 5-aminolevulinic Acid are Involved in Regulation of Stomatal Opening in Apple Leaves

LIU Long-bo,AN Yu-yan,XIONG Li-jun,and WANG Liang-ju*   

  1. (College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China)
  • Online:2016-05-25 Published:2016-05-25

摘要:

以苹果离体叶片为材料,发现外源5-ALA处理可以诱导下表皮保卫细胞内黄酮醇含量显著上升。在5-ALA预处理抑制外源ABA诱导气孔关闭的同时,叶片保卫细胞ROS含量下降,而用外源槲皮素及山奈酚等黄酮醇预处理,也抑制了ABA诱导的苹果叶片气孔关闭,并且降低保卫细胞ROS含量。此外,槲皮素和山奈酚可以抑制外源H2O2诱导的苹果叶片气孔关闭。以上结果喻示,5-ALA诱导苹果叶片气孔开放与其上调保卫细胞中黄酮醇含量而后降低活性氧含量有关。

关键词: 苹果, ABA, 5-ALA, 黄酮醇, 气孔开度, 保卫细胞

Abstract:

Using diphenylboric acid 2-amino ethyl esterDPBA),a flavonol fluorescent dyeand a laser scanning confocal microscopeLSCM),we found that an increase of flavonol fluorescence appeared in the guard cells of the abaxial epidermis of detached leaves of appleMalus ´ domestica Korkh.Fuji’),especially around the nucleusafter 5-ALA pretreatment for 24 h. 5-ALA pretreatment also inhibited ABA-induced stomatal closurewhich was inseparably related to the reduction of the reaction oxygen speciesROSaccumulation. Coincidentlypretreatment of quercetin or kaempferoltwo kinds of natural flavonolsalso inhibited stomatal closure induced by ABAand meanwhile decreased ROS content in the guard cells of apple leaves. Furthermorethe exogenous flavonols eliminated the effect of H2O2 that induced stomatal closure in apple leaves. Together with these aboveit can be deduced that 5-ALA-induced stomatal opening is associated with its up-regulation of flavonol levels in guard cellswhich then eliminates ROS levels. Thereforeflavonols are involved in stomatal regulation induced by 5-ALA in apple leaves.

Key words: apple, ABA, 5-ALA, flavonol, stomatal aperture, guard cell

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