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园艺学报 ›› 2016, Vol. 43 ›› Issue (3): 595-602.doi: 10.16420/j.issn.0513-353x.2015-0787

• 研究报告 • 上一篇    下一篇

双孢蘑菇POD酶学特性及其与褐变的关系

吕 蔷,朱继英,王相友*   

  1. 山东理工大学农业工程与食品科学学院,山东淄博 255049
  • 出版日期:2016-03-25 发布日期:2016-03-25
  • 基金资助:

    山东省农业重大应用技术创新课题;国家自然科学基金项目(31301819)

Characteristics of Peroxidase from Agaricus bisporus and Its Relationship with Browning

Lü Qiang,ZHU Ji-ying,and WANG Xiang-you*   

  1. School of Agricultural and Food Engineering,Shandong University of Technology,Zibo,Shandong 255049,China
  • Online:2016-03-25 Published:2016-03-25

摘要:

为了研究POD在双孢蘑菇褐变过程中的作用,对POD酶学特性进行了测定,并对不同贮藏条件下双孢蘑菇中POD活性变化与褐变的关系进行了分析。结果表明:以愈创木酚为底物时,双孢蘑菇中POD的米氏常数(Km)为5.704 mmol ? L-1,最适反应pH为5.0,最适反应温度为45 ℃;低温可以显著抑制POD的活性,20 ℃和10 ℃时其相对酶活性分别为68%和55%,2 ~10 ℃范围内的酶活性差异不显著;20 ℃贮藏1 d和2 d时POD酶活分别是10 ℃贮藏的4.8倍和10.9倍。7种化合物对POD活性的抑制作用由强到弱依次为:DTT > AsA > L-Cys > SDS > 无水亚硫酸钠 > 柠檬酸 > NaCl。贮藏过程中,虽然POD抑制剂处理双孢蘑菇的褐变程度和气调处理没有显著性差异,但其POD活性显著高于气调处理,说明POD活性高不是引起双孢蘑菇组织褐变的主要因素。

关键词: 双孢蘑菇, 过氧化物酶, 酶学特性, 贮藏

Abstract:

In order to study the effects of peroxidase(POD)on browning of Agaricus bisporus,the POD enzymatic characteristics and the relationship of POD activity and browning were analyzed. Results showed that the Michaelis constant Km of POD was 5.704 mmol ? L-1,and the appropriate pH and reaction temperature were 5.0 and 45 ℃,respectively,under the condition of guaiacol as the substrate. Low temperature could significantly inhibit POD activity,the relative activities of POD at 20 ℃ and 10 ℃ were 68% and 55%,respectively,while there was no significant difference of POD activities from 2 ℃ to 10 ℃. Compared with 10 ℃,the POD activities of Agaricus bisporus under 20 ℃ at 1 and 2 d were increased by 4.8 and 10.9 fold,respectively. Dithiothreitol(DTT)had the strongest inhibition on POD activity,followed by ascorbic acid(AsA),L-cysteine(L-Cys),sodium lauryl sulfate(SDS),anhydrous sodium sulfite,citric acid and NaCl. During storage,there was no significant difference in the browning degree between Agaricus bisporus treated by inhibitor and controlled atmospheres,while POD activity of Agaricus bisporus treated by inhibitor was much higher,which indicated that high POD activity was not the main factor for Agaricus bisporus browning.

Key words: Agaricus bisporus, peroxidase, enzymatic characteristic, storage

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