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园艺学报 ›› 2009, Vol. 36 ›› Issue (9): 1255-1260.

• 果树 • 上一篇    下一篇

苹果MdFT基因对番茄的遗传转化

李伟明1,2;王双双1;姚玉新1;赵长增2;郝玉金1;由春香1*   

  1. (1山东农业大学作物生物学国家重点实验室, 园艺科学与工程学院, 山东泰安271018; 2 肃农业大学农学院园艺系, 兰州730070)
  • 收稿日期:2009-04-10 修回日期:2009-07-14 出版日期:2009-09-25 发布日期:2009-09-25
  • 通讯作者: 由春香

Genetic Transformation of Apple MdFT Gene into Tomato

LIWei-ming1,2;WANG Shuang-shuang1;YAO Yu-xin1;ZHAO Chang-zeng2;HAO Yu-jin1;YOU Chun-xiang1*   

  1. (1State Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University,Tai'an, Shandong 271018, China; 2Department of Horticulture, College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China)
  • Received:2009-04-10 Revised:2009-07-14 Online:2009-09-25 Published:2009-09-25
  • Contact: YOU Chun-xiang

摘要: 通过RT2PCR扩增, 从苹果叶片cDNA中克隆了FT基因的同源基因MdFT, 构建了花椰菜病毒35S启动子驱动的MdFT植物表达载体35S: : MdFT, 并利用根癌农杆菌介导法将其导入番茄栽培品种‘中蔬四号’; 同时转化拟南芥A tFT基因作为阳性对照。从添加卡那霉素的筛选培养基上再生了抗性植株,PCR扩增证明, 外源基因MdFTAtFT已经整合到转基因番茄的基因组, 半定量RT-PCR则证明它们已经在转基因番茄中得到异位过量表达。形态鉴定发现, 转基因番茄植株比非转基因对照植株开花早, 表明成功地从苹果中克隆了成花素基因MdFT, 该基因具有通过转基因缩短苹果树童期的潜在价值。

关键词: 苹果, FT基因, 异位过量表达, 番茄

Abstract: It is of great importance for field yield of fruit trees to generate flower buds. Recently, FT protein has been proved to be a florigen in higher plants. In this study, full-length cDNA of MdFT gene, a homolog of Arabidopsis AtFT, was amplified from apple leaf cDNAs with RT-PCR. Subsequently, a plant expression construct containing MdFT gene driven by cauliflowermosaic virus 35S promoter was obtained and introduced into tomato cultivar ‘Zhongshu 4 ’with Agrobacterium-mediated transformation. In parallel, Arabidopsis AtFT gene was transformed as a positive control. Finally, transgenic tomato lineswere regenerated from selection medium plus kanamycin. PCR amp lification verified the integration of exogenous genes into the host genome, and semiquantitative RT-PCR disp layed their ectopic overexpression in transgenic tomatoes. Furthermore, morphological observation found that transgenic tomato lines flowered earlier than the non-transgenic control, suggestive of our successful cloning of florigen gene MdFT from apple, which has the
potential as a target gene to shorten juvenility in apple tree.

Key words: apple, FT gene, ectopic overexpression, tomato

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