关键词: 草莓, 果实, 脱落酸, 蔗糖, FaASR基因

Abstract:

In this study，we used Sweet Charlie strawberry as test materials，based on cloning the strawberries ASR homologous gene，we carried out the bioinformatics and temporal expression analysis of FaASR，by manipulating ASR gene expression level in strawberry fruit，we tested the changes of physiological indicators，including phenotypic changes，sugar，ABA，pigments content，and fruit firmness. In addition，we measured the expression changes of some anthocyanin-related gene，such as CHS and UFGT，by which we revealed the regulation mechanisms of ASR gene over strawberry fruit ripening. Strawberry ASR contained a typical domain of ABA/WDS that was related to fruit ripening and stress-resistance，and ASR gene over-expression could promote strawberry fruit coloring，endogenous ABA and sucrose accumulation，fruit softening，and induced the transcription levels of anthocyanin related genes CHS and UFGT. The present study will further reveal the molecular mechanisms of information transmission in fruit development，and will also lay an important foundation for future molecular improvement of strawberries breeding.

Key words: strawberry, fruit, ABA, sucrose, FaASR