https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2008, Vol. 35 ›› Issue (6): 819-826.

• 蔬菜 • 上一篇    下一篇

硅对菜薹炭疽病防御反应中信号物质的影响

杨暹1*;杨跃生2;冯红贤1   

  1. 1华南农业大学园艺学院,广州 510642;2华南农业大学生命科学学院,广州 510642)
  • 收稿日期:2008-01-02 修回日期:2008-05-12 出版日期:2008-06-25 发布日期:2008-06-25
  • 通讯作者: 杨暹

The Effects of Silicon on Signal Substances of Anthracnose-Defense Response in Flowering Chinese Cabbage

YANG Xian1*,YANG Yue-sheng2,and FENG Hong-xian1   

  1. (1College of Horticulture, South China Agricultural University, Guangzhou 510642, China ; 2College of Life Sciences, South China Agricultural University, Guangzhou 510642, China)
  • Received:2008-01-02 Revised:2008-05-12 Online:2008-06-25 Published:2008-06-25
  • Contact: YANG Xian

摘要:

通过不同硅(Si)处理和接种炭疽病菌研究Si对菜薹肌醇磷脂信号系统和钙调素信号系统的影响。结果表明:Si对磷脂酰肌醇(PI)和磷脂酰肌醇-4-磷酸(PIP)的形成有明显的调节作用。感染炭疽病后,不同基因型的PI消长明显不同,适宜Si水平下感病后可诱导形成较高水平的PI。感染炭疽病菌后感病品种的中Si处理和抗病品种的低Si、中Si处理的PI呈明显增长趋势,而其它处理有消有长。感病品种在感病前期有抑制PIP产生的趋势,而后期转为促进作用,但抗病品种的变化规律与感病品种相反。在适宜Si水平下感染炭疽病菌后可明显提高抗、感病品种PIP含量,特别是在感病后期其诱导作用更明显,这可能与PI后期大量降解,传递抗病信息有关。Si影响钙调素(CaM)含量,其影响程度与炭疽病菌的作用有关。低Si水平明显促进了炭疽病菌对感病品种CaM的诱导作用,以中Si水平对抗病品种的诱导促进作用相对较强,而且感病品种的诱导促进作用比抗病品种明显。感病品种的中Si处理与抗病品种的低Si处理在感病后的CaM变化相对缓和。表明CaM参与炭疽病致病过程的调控。

关键词: 菜薹, 硅, 炭疽病, 肌醇磷脂, 钙调素

Abstract:

The influences of silicon (Si) on the signaling systems of phosphoinositides and calmodulin (CaM) in flowering Chinese cabbage (Brassica Campestris L. ssp. Chinensis var. utilis Tsen et Lee ) was studied using the inoculation of anthracnose (Colletotrichum higginsianum) at different Si levels. Results indicated that Si played a manifest regulating role on the formation of phosphatidylinositol (PI) and phosphatidylinositol-4-monophosphate (PIP). After the infection by anthracnose, there were clearly different fluctuations of PI contents between different cultivars . At the suitable Si level, infection induced high PI content. Due to the infection, PI content increased manifestly in the susceptible cultivar at medium Si level and in the resistant cultivar at low and medium Si levels, but showed unstable trend in other treatments. The accumulation of PIP was inhibited at the early infection stage and turned to be promoted at the late infection stage in the susceptible cultivar, but the accumulation pattern of PIP was completely reverse in the resistant cultivar. At suitable Si level, the infection by anthracnose manifestly increased PIP contents in both susceptible and resistant cultivars, especially in the late infection stage with stronger induction. This is probably related to the degradation of PI in large amount and the transfer of disease-resisting signals at the late infection stage. Si also affected the CaM contents, with the extents relating to the infection by anthracnose. Low Si level clearly promoted the inducing effect of anthracnose on CaM in the susceptible cultivar, while medium Si level showed strong promoting effect on the
收稿日期:2008-01-02 ;修回日期:2008-

Key words: Brassica campestris L. ssp. Chinensis var. utilis Tsen et Lee, silicon, Colletotrichum higginsianum, phosphoinositide, calmodulin

中图分类号: