https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2015, Vol. 42 ›› Issue (11): 2123-2132.doi: 10.16420/j.issn.0513-353x.2015-0290

• 果树 • 上一篇    下一篇

采前除袋铺设反光膜对桃果实着色及相关基因#br# 表达的影响

马瑞娟*,张斌斌,张春华,蔡志翔,颜志梅   

  1. 江苏省农业科学院园艺研究所,江苏省高效园艺作物遗传改良重点实验室,南京 210014
  • 出版日期:2015-11-25 发布日期:2015-11-25
  • 基金资助:

    国家现代农业产业技术体系建设专项资金项目(CARS-31);江苏省农业科技自主创新资金项目[CX(14)2015]

Effect of Bag Removing with Reflective Film Mulching Before Harvest on#br# Fruit Coloration and Expression of Anthocyanin Related Genes in Peach

MA Rui-juan*,ZHANG Bin-bin,ZHANG Chun-hua,CAI Zhi-xiang,and YAN Zhi-mei   

  1. Institute of Horticulture,Jiangsu Academy of Agricultural Sciences,Jiangsu Key Laboratory for Horticultural Crop
    Genetic Improvement,Nanjing 210014,China
  • Online:2015-11-25 Published:2015-11-25

摘要: 为探讨采前铺设反光膜对桃果实外观品质的影响及着色机制,以容易着色的晚熟桃品种‘霞 晖8 号’为试材,采收前12 d 除去外黄内黑双层袋,研究了不套袋不铺反光膜、除袋不铺反光膜和除袋 铺反光膜对果皮色泽、色素含量以及与果实着色相关的酶及其基因表达的影响。结果表明:除袋不铺反 光膜和除袋铺反光膜均能显著提高果实红绿色差值(a*)、红绿色差/黄蓝色差值(a*/b*)、色饱和度(C) 和果皮花色素苷含量,降低色调角(h),除袋铺反光膜较除袋不铺反光膜处理花色素苷含量更高(P < 0.05); 花色素苷合成相关基因UFGT 和CHS 在试验之初即上调表达,而DFR、LDOX 和F3H 则随试验的进行而 上调表达。此外,不套袋不铺反光膜处理的苯丙氨酸解氨酶(PAL)活性逐渐降低,而其他两个处理呈先 升高后下降趋势,且采前除袋铺反光膜较除袋不铺反光膜的处理活性更高。据此推测,除袋不铺反光膜 和除袋铺反光膜条件下果皮花色素苷的合成是多个基因协同调控作用的结果,尤以除袋铺反光膜处理促 进花色素苷积累的效果更明显。

关键词: 桃, 着色, 反光膜, 色差, 色素, 基因表达

Abstract:

This study explored the effects of applying reflective film before harvest on fruit
appearance quality of peach(Prunus persica),and discussed the influencing mechanism on fruit coloration.
Yellow outside and black inside double-layer bags were removed from fruit 12 d before harvest. Peel
color,pigment content,enzyme activity as well as the gene expression related to fruit coloration of a
well-colored and late-ripening peach cultivar Xiahui 8 with different treatments(non-bagging with
non-reflective film mulching,debagging with non-reflective film mulching,debagging with reflective film
mulching)were researched. The results showed that both debagging with non-reflective film mulching and
debagging with reflective film mulching could significantly improve red and green color difference value(a*),a*/b*(b* is yellow and blue color difference value),chroma(C),anthocyanin(Ant)content and
reduce hue angle(h),while debagging with reflective film mulching had higher Ant content than
debagging with non-reflective film mulching(P < 0.05). The two treatments up-regulated the transcript level
of anthocyanin biosynthetic related genes(UFGT,CHS)at the beginning of the experiment,however,
the expression of DFR,LDOX and F3H were enhanced along with the testing process. A downward trend
on phenylalanine ammonia lyase(PAL)activity of non-bagging with non-reflective film mulching was
observed during the experiment,however,the peel PAL activity of the other two treatments first increased
and then decreased. What’s more,peel PAL activity of debagging with reflective film mulching was higher
than debagging with non-reflective film mulching. These results suggested that Ant synthesis in the peel
was the result of cooperation among several genes,and debagging with reflective film mulching had better
effect on promoting Ant accumulation than debagging with non-reflective film mulching.

Key words: peach, coloration, reflective film, color difference, pigment, gene expression

中图分类号: