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园艺学报 ›› 2015, Vol. 42 ›› Issue (6): 1121-1128.doi: 10.16420/j.issn.0513-353x.2015-0024

• 蔬菜 • 上一篇    下一篇

甜瓜‘PMR6’抗白粉病基因的遗传及其定位研究

卢浩,王贤磊,高兴旺,宁雪飞,陈静,李冠   

  1. 新疆大学生命科学与技术学院,乌鲁木齐 830046
  • 出版日期:2015-06-25 发布日期:2015-06-25
  • 基金资助:

    国家自然科学基金项目(31260258)

Inheritance and Location of Powdery Mildew Resistance Gene in Melon PMR6

LU Hao, WANG Xian-Lei, GAO Xing-Wang, NING Xue-Fei, CHEN Jing, LI Guan   

  1. College of Life Science and Technology,Xinjiang University,Urumqi 830046,China
  • Online:2015-06-25 Published:2015-06-25

摘要: 以甜瓜(Cucumis melo L.)感白粉病品种‘Hami413’为受体亲本,抗白粉病品种‘PMR6’为供体亲本构建的255株BC2分离群体为材料,研究‘PMR6’抗白粉病的遗传规律及基因定位。群体遗传分析表明,BC2群体中对白粉病菌Podosphaera xanthii生理小种1的抗性由显性单基因控制,基因命名为Pm-PMR6-1;利用混合分组分析法(Bulked segregant analysis,BSA)从分布于甜瓜12个连锁群上的390个SSR分子标记中筛选出5个多态性标记;通过连锁分析,将该抗性基因定位于12号连锁群SSR标记DM0191与CMBR111之间;根据甜瓜基因组信息设计SSR引物,进一步将该基因定位于SSR12407与SSR12202之间,并且该抗性基因与标记Mu7191共分离;比对基因组序列,两标记间物理距离约226 kb,预测35个候选基因。

关键词: 甜瓜, Podosphaera xanthii 生理小种1, SSR, 抗性基因

Abstract: To identify the chromosomal location associated with powdery mildew resistance gene,the segregation of resistance was evaluated in BC2 population derived from a cross between the resistance melon PMR6 and the susceptible melon Hami413. Genetic analysis showed that the resistance to Podosphaera xanthii race 1 was controlled by one single dominant gene in BC2 population,and the gene was named Pm-PMR6-1. Among 390 simple sequence repeat(SSR)markers distributed on 12 linkage groups,5 polymorphic markers were identified by Bulked segregant analysis(BSA). The resistance gene was located on linkage group Ⅻ between SSR markers SSR12407 and SSR12202,and the physical distance was 226 kb according to the melon genome database. Thirty-five candidate genes were predicted in this region. The resistance gene Pm-PMR6-1 co-segregated with SSR marker Mu7191 in 255 BC2 progeny. The candidate genes in this region provide a potential target site for further fine mapping and cloning of powdery mildew resistance gene in melon PMR6.

Key words: Cucumis melo, Podosphaera xanthii race 1, SSR, resistance gene

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