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园艺学报 ›› 2008, Vol. 35 ›› Issue (6): 879-884.

• 研究简报 • 上一篇    下一篇

青花菜预冷后乙烯生成特性及受体基因的表达分析

王 然1*;王成荣1;秦斐斐1;加藤雅也2;西川芙美惠2;兵藤宏2   

  1. 1青岛农业大学园艺学院,山东青岛,266109; 2日本静冈大学农学部,日本静冈市大谷836)
  • 收稿日期:2008-04-01 修回日期:2008-05-09 出版日期:2008-06-25 发布日期:2008-06-25
  • 通讯作者: 王 然

Characteristics of Ethylene Production and Gene Expression of Ethylene Receptors in Pre-cooled Broccoli (Brassica oleracea L. var. italica)

WANG Ran1*, WANG Cheng-rong1,QIN Fei-fei1, Masaya Kato2, Fumie Nishikawa2, and Hiroshi Hyodo2   

  1. (1Department of Horticultur,, Qingdao Agricultural University, Qingdao, Shandong, 266109, China; 2Faculty of Agriculture, Shizuoka University, 836 Ohya, Shizuoka422-8529, Japan)
  • Received:2008-04-01 Revised:2008-05-09 Online:2008-06-25 Published:2008-06-25
  • Contact: WANG Ran

摘要: 研究了采收的青花菜预冷后置于20 ℃下早期乙烯生成特性和乙烯受体基因的表达。结果表明,乙烯生成速率在茎切面第一层0~6 h明显上升,在小花中明显下降。ACC合成酶活性与乙烯生成速率的变化一致。ACC含量在各组织中为先上升后下降,以小花中最低。MACC含量以花头基部最低,小花中较高,变化平稳。各组织中ACC氧化酶活性在0~12 h呈增加趋势,但小花中的ACC氧化酶活性明显高于茎组织,与乙烯生成速率变化不一致。Northern杂交分析表明,茎切面第一层的BO-ACS1 和BO-ACO2的 mRNA水平在0 h高于小花,12 h 时mRNA的水平低于0 h。小花中BO-ACS1 mRNA在12 h增加不明显, BO-ACO2 mRNA增加明显。BO-ETR1、BO-ERSBO-ETR2在茎切面第一层和BO-ERS在小花中的转录物12 h时均比0 h有不同程度的增加,BO-ETR2 mRNA仅在茎组织中检测到。

关键词: 青花菜, 乙烯, 乙烯受体, 基因表达

Abstract:

The characteristic of ethylene production and gene expression of ethylene receptors were studied in pre-cooled broccoli which were stored under 20 ℃. The results showed that during 0-6 h of storage, the ethylene production increased in the cross section of stem (0-2 mm), while decreased markedly in florets. Changes in the activity of ACC synthase were in agreement with those of the ethylene production rate. ACC content increased from 0 h to 6 h and decreased from 6 h to 12 h in all tested tissues. The florets had the lowest level of ACC content. The MACC level in florets was relatively high and remained stable during the experimental period. The lowest level of MACC appeared in the basal portion of florets. Though it was significantly higher in florets than that in the stem tissues, the activity of ACC oxidase increased from 0 h to 12 h in all tested tissues,which was inconsistent with the ethylene production. Northern blot analysis showed that mRNA levels of BO-ACS1 and BO-ACO2 in stem tissues were higher than that in florets at 0 h; mRNA levels at 12 h were lower than that at 0 h. In florets, the mRNA level of BO-ACS1 didn't increase at 12 h, however mRNA level of BO-ACO2 increased significantly. The transcripts of BO-ETR1, BO-ERS, and BO-ETR2 in the first layer of the cross section of stem and the BO-ERS in the florets were higher at 12 h than that at 0 h. The mRNA of BO-ETR2 could be only detected in the stem tissues.

Key words: broccoli, ethylene, ethylene receptor, gene expression

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