关键词: 风信子, 花色, 花色苷, 液质联用

Abstract: The anthocyanin profiles in 12 cultivars of hyacinthus（Hyacinthus orientalis L.）were analyzed. The flower color was observed according to the Royal Horticultural Society Color Card （RHSCC）. Petal pigment type was identified by characteristic color reaction. UPLC-PAD and UPLC-Q-TOF-MS were used for qualitative and quantitative analysis of anthocyanins. The results showed that colored carotenoids in petals of all cultivars were not detected，by contrast，every cultivar，except for‘City of Haarlem’and‘Aiolos’，contained anthocyanins in petals. Among ten anthocyanin-contained cultivars，‘Woodstock’contained the highest anthocyanin content，followed by‘Jan Bos’，‘Shangnong Zaofen’，and‘Shangnong Zihong’，respectively. Totally 9 anthocyanin components were detected in petals of these cultivars，including Pelargonidin 3-O-glucoside，Pelargonidin 3-O-glucoside-5-O-malonylglucoside，Cyanidin 3-O-coumaroylglucoside-5-O-glucoside，Pelargonidin 3-O-coumaroyl-glucoside-5-O-glucoside，Pelargonidin 3-O-coumaroylglucoside-5-O-acetyglucoside，Cyanidin 3-O-coumaroylglucoside-5-O- malonylglucoside，Pelargonidin 3-O-coumaroylglucoside-5-O-malonylglucoside，Pelargonidin3-O- feruloylglucoside-5-O-malonylglucoside，and Pelargonidin 3-O-caffeoylglucoside-5-O-malonylglucoside.‘Shangnong Zaofen’，‘Shangnong Zhongfen’，‘Gipsy Queen’，and‘Marconi’contained only pelargonidin derivated anthocyanins in petals while other six cultivars contained majorly pelargonidin derivated anthocyanins and some cyanidin derivated anthocyanins in petals. Pelargonidin 3-O- coumaroylglucoside-5-O-malonylglucoside was the dominant anthocyanin in‘Shangnong Zaofen’，‘Shangnong Zhongfen’，‘Gipsy Queen’，‘Jan Bos’，‘Marconi’，and‘Lady Derby’，while Pelargonidin 3-O-coumaroylglucoside-5-O-acetyglucoside was the dominant anthocyanin in‘Anna Lisa’and ‘Woodstock’；While，Cyanidin 3-O-coumaroylglucoside-5-O-malonylglucoside and Pelargonidin 3-O-coumaroylglucoside-5-O-glucoside was the dominant anthocyanin in‘Shangnong Zihong’and‘Sky Line’，respectively.

Key words: Hyacinthus orientalis, flower color, anthocyanin, UPLC-MS