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园艺学报 ›› 2014, Vol. 41 ›› Issue (12): 2497-2506.

• 研究报告 • 上一篇    下一篇

辣椒种内遗传图谱的构建及主要农艺性状的QTL分析

段蒙蒙,王 宁,毛胜利,张正海,王立浩,张宝玺   

  1. 中国农业科学院蔬菜花卉研究所,北京 100081
  • 出版日期:2014-12-25 发布日期:2014-12-25
  • 基金资助:

    国家‘863’计划项目(2012AA100103002);国家科技支撑计划项目(2011BAZ01732);农业部农业科技产业计划项目(CARS-25);国家公益性研究所项目(ICS,CAAS)(1610032011011)

Construction of the Intraspecific Genetic Linkage Map and QTL Analysis for Main Agronomic Traits of Capsicum annuum

DUAN Meng-Meng, WANG Ning, MAO Sheng-Li, ZHANG Zheng-Hai, WANG Li-Hao, ZHANG Bao-Xi   

  1. Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China
  • Online:2014-12-25 Published:2014-12-25

摘要: 以一年生辣椒(Capsicum annuum L.)‘Perennial’、‘83-58’为亲本构建的包含128个株系的F6代重组自交系为作图群体,构建了一个包含有18个连锁群的遗传图谱。该遗传图谱共有131个标记,其中SSR标记105个,CAPS标记23个,SCAR标记1个,果实辣味(pun)和果柄着生方向(up)2个形态标记。该图谱全长633.71 cM,标记间的平均距离为4.8 cM,连锁群的长度为7.07 ~ 75.16 cM,平均长度为35.21 cM,每个连锁群上的标记数为3 ~ 17个。对群体进行10个主要农艺性状调查,利用基于完备区间作图方法(Inclusive Composite Interval Mapping)的QTL IciMapping V3.2软件(http://www. isbreeding. net/)选择加性效应模型ICIM-ADD进行QTL定位,在重组自交系(RIL)群体中共检测到株高、伸展度、始花节位、叶长、叶宽、侧枝长度、果形、果形指数、心室数和果实横径等10个性状的20个QTL位点。

关键词: 辣椒, 遗传图谱, 农艺学性状, QTL

Abstract: A intraspecific linkage map of Capsicum annuum was constructed from a recombinant inbred line population of F6 generation with 128 plants,which derived from a small fruit-sized hot pepper line‘Perennial’and a large fruit-sized sweet pepper line‘83-58’. In this map,18 linkage groups were generated with 131 markers(105 SSR markers,23 CAPS markers,1 SCAR marker and 2 morphological markers). This genetic map covered 633.71 cM with an average intermarker distance of 4.8 cM. The length of linkage groups ranged from 7.07 to 75.16 cM with 3 to 17 markers per linkage group,and the average length reached 35.21 cM. Ten main agronomic taits of this population were collected for QTL mapping with QTL IciMapping V3.2 software,which was based on inclusive composite interval mappingmethod,and an additive effect model ICIM-ADD was selected in the QTL mapping. A total of 20 QTLs of 10 traits(stem length,plant elongation,first flower node,leaf length,leaf width,lateral bud,fruit shape,fruit shape index,fruit width,number of node)were mapped on the genetic map.

Key words: pepper, genetic linkage map, agronomic traits, QTL, RIL

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