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园艺学报 ›› 2014, Vol. 41 ›› Issue (12): 2373-2382.

• 果树 •    下一篇

叶用银杏种质资源黄酮和萜内酯类含量及AFLP遗传多样性分析

吴岐奎,邢世岩*,王 萱,孙立民   

  1. 山东农业大学林学院,山东泰安 271018
  • 出版日期:2014-12-25 发布日期:2014-12-25
  • 基金资助:

    国家科技支撑计划项目(2012BAD21B04);山东省农业良种工程重大课题(鲁农良字[2011]7号);国家植物种质资源共享平台项目(2013-39)

Genetic Diversity of Leaf-used Ginkgo biloba Germplasms Based on Flavonoids and Ginkgolides Contents and AFLP Markers

WU Qi-Kui, XING Shi-Yan-*, WANG Xuan, SUN Li-Min   

  1. College of Forestry,Shandong Agricultural University,Tai’an,Shandong 271018,China
  • Online:2014-12-25 Published:2014-12-25

摘要: 对叶用银杏种质资源进行黄酮和萜内酯类含量及AFLP遗传关系分析。结果表明,叶用银杏种质资源在黄酮和萜内酯类含量水平和DNA分子水平上都存在较高的遗传多样性。68份叶用银杏种质叶片提取物成分中银杏苦内酯A、B、C、J和白果内酯B,银杏总内酯,黄酮的平均含量分别为0.0371%、0.0185%、0.0280%、0.0133%、0.0243%、0.1216%、1.7103%。8对AFLP引物组合共扩增出1 408条谱带,其中多态带为99.28%,Nei’s基因多样性和Shannon’s指数分别为0.1939和0.3142;种质间的遗传相似系数在0.2924 ~ 0.7413之间,平均值为0.4888。种质间基于黄酮和萜内酯类含量分析的聚类分析结果与基于遗传相似系数的UPGMA聚类分析结果基本一致。利用黄酮和萜内酯类含量和AFLP分析相结合的方法,筛选出高黄酮和萜内酯类特异种质,对叶用银杏种质资源的评价与保护有一定的积极意义。

关键词: 银杏, 种质资源, 叶用, 黄酮, 萜内酯类, 含量, AFLP, 遗传多样性

Abstract: The genetic relationship of leaf-used Ginkgo biloba L. germplasms were evaluated by flavonoids and ginkgolides contents and AFLP markers. The results showed that rich genetic diversity existed in both flavonoids and ginkgolides contents and AFLP markers. The average content of leaf extracts(Ga,Gb,Gc,Gj,Bb,Te,GF)in 68 leaf-used G. biloba L. germplasms are 0.0371%,0.0185%,0.0280%,0.0133%,0.0243%,0.1216% and 1.7103% respectively. Among the 1 408 bands obtained from 8 selective primer pairs,99.28% of them were polymorphic. Nei’s genetic diversity was 0.1939,and Shannon’s index was 0.3142. The molecular genetic similarity coefficients range from 0.2924 to 0.7413,with an average of 0.4888. The results of hierarchical cluster analysis based the flavonoids and ginkgolides contents of different populations and UPGMA exhibited a good consistency. By the hybrid method of flavonoids and ginkgolides contents and AFLP markers analysis,screen out the distinctive G. biloba germplasms of high-flavonoids and ginkgolides,would be valuable for germplasm management and utilization of leaf-used G. biloba L. germplasm.

Key words: Ginkgo biloba, germplasm, leaf-used, flavonoids, ginkgolides, content, AFLP, genetic diversity

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