https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2014, Vol. 41 ›› Issue (11): 2281-2390.

• 观赏植物 • 上一篇    下一篇

换锦花LsMYB4基因的克隆与表达分析

许振渊,高燕会*,周芬静,李国庆,熊艳,童再康   

  1. 浙江农林大学亚热带森林培育国家重点实验室培育基地,浙江临安 311300
  • 收稿日期:2014-06-03 出版日期:2014-11-25 发布日期:2014-11-25
  • 基金资助:
    浙江省花卉新品种选育重大科技专项重点项目(2012C12909-14)

Cloning and Expression Analysis of LsMYB4 Gene in Lycoris sprengeri

XU Zhen-yuan,GAO Yan-hui*,ZHOU Fen-jing,LI Guo-qing,XIONG Yan,and TONG Zai-kang   

  1. The Nurturing Station for the State Key Laboratory of Subtropical Silviculture,Zhejiang Agriculture and Forestry University,Lin’an,Zhejiang 311300,China
  • Received:2014-06-03 Online:2014-11-25 Published:2014-11-25

摘要: 以换锦花(Lycoris sprengeri)为材料,采用RT-PCR方法和RACE技术相结合的方法,从花瓣中克隆了MYB基因的cDNA全长序列,命名为LsMYB4。该序列全长793 bp,包含606 bp完整开放阅读框,编码201个氨基酸,具有明显的R2R3-MYB结构域,与中国水仙NtMYB相似性达96%。实时荧光定量PCR分析结果表明,LsMYB4在换锦花不同组织和不同花期均有表达,其中在花瓣中的相对表达量比叶中多9 ~ 10倍,在盛花期的相对表达量比花苞期多20倍。不同花色无性系花瓣中表达量存在差异,花色较浅的无性系高于其它无性系,推测LsMYB4在调控换锦花花色形成的过程中起着负调控作用。

关键词: 换锦花, MYB基因, 克隆, 表达分析

Abstract: A MYB gene named LsMYB4 was cloned by RT-PCR and RACE methods from petals of Lycoris sprengeri. Sequences analysis showed that the full-length MYB cDNA was 793 bp and contained a ORF of 606 bp encoding 201 amino acids. The LsMYB4 protein had a conserved R2R3-MYB domain and the amino acids sequence shared up to 96% homelogies with anthycyanin biosynthesis-related R2R3-MYB transcription factor in Narcissus tazetta. The expression analysis by real time qRT-PCR showed that LsMYB4 was expressed in different tissues and flowering periods. The expression level in petal was almost ten times higher than in leaf. And in florescence period the expression of LsMYB4 was twenty times higher than in early budding period. The LsMYB4 expressed discrepantly in four clones of L. sprengeri,and results of it implicated that the lighter flower color,the higher expression level. This showed that LsMYB4 played a possibly negative role in anthocyanin biosynthesis of Lycoris sprengeri.

Key words: Lycoris sprengeri, MYB, cloning, expression analysis

中图分类号: