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园艺学报 ›› 2014, Vol. 41 ›› Issue (10): 1993-2000.

• 果树 • 上一篇    下一篇

嫁接嵌合体‘早红’脐橙果实细胞来源鉴定及其糖酸和香气物质分析

吴巨勋1,肖慧玲2,王小花1,伊华林1,*   

  1. 1 华中农业大学园艺林学学院,园艺植物生物学教育部重点实验室,武汉 430070;2 湖北工程学院城市建设学院,湖北孝感 432000
  • 收稿日期:2014-07-23 出版日期:2014-10-25 发布日期:2014-10-25
  • 基金资助:

    国家现代农业产业技术体系建设专项资金项目(CARS-27);国家科技支撑计划课题(2013BAD021302);教育部创新团队发展计划项目(IRT13065)

Identification of Fruit Cell Source of Graft Chimera‘Zaohong’Navel Orange and Analysis of Soluble Sugars,Organic Acids and Aromas of Fruit

WU Ju-xun1,XIAO Hui-ling2,WANG Xiao-hua1,and YI Hua-lin1,*   

  1. 1College of Horticulture and Forestry Science,Huazhong Agricultural University,the Education Ministry Key Laboratory of Horticultural Plant Biology,Wuhan 430070,China;2 College of Urban Construction,Hubei Engineering University,Xiaogan,Hubei 432000,China
  • Received:2014-07-23 Online:2014-10-25 Published:2014-10-25

摘要: ‘早红’脐橙[Citrus sinensis(L.)Osbeck + C. unshiu Marc.]是来自‘罗伯逊’脐橙[C. sinensis (L.)Osbeck]和‘国庆1号’温州蜜柑(C. unshiu Marc.)的嫁接嵌合体。采用SSR和CpSSR分子标记分析了‘早红’脐橙的叶片、汁胞、囊瓣壁和白皮层的细胞来源,采用气相色谱(GC)和顶空固相微萃取—气质联用(HS–SPME–GC–MS)的方法测定了‘早红’脐橙及其两个嫁接亲本的可溶性糖和有机酸含量,以及果皮和果肉的香气物质。结果表明,‘早红’脐橙汁胞和囊瓣壁中均含有两个亲本的遗传物质,果肉中的有机酸含量显著低于两个亲本,果皮中的香气物质与‘罗伯逊’脐橙果皮基本一致,但是果肉中的香气物质与两个亲本都不尽相同。

关键词: 脐橙, 嫁接嵌合体, SSR, CpSSR, 可溶性糖, 有机酸, 香气

Abstract: ‘Zaohong’navel orange[Citrus sinensis(L.)Osbeck + C. unshiu Marc.] is a graft chimaera from‘Robertson’navel orange[C. sinensis(L.)Osbeck] and‘Guoqing 1’satsuma mandarin(C. unshiu Marc.). The cell sources of leaf,juice sac,segment wall and albedo of‘Zaohong’navel orange were analyzed using SSR and CpSSR. Gas chromatography(GC)and headspace solid-phase microextraction–GC–MS(HS–SPME–GC–MS)were used to measure the content of soluble sugars and organic acids in the pulps of‘Zaohong’navel orange and its grafting parents,and aromas in the peels and pulps of these three materials,respectively. Results revealed that,juice sac and segment wall of ‘Zaohong’navel orange contained two genotype cells from two parents,the content of organic acids in its pulp was significantly lower than that in the pulps of two parents,the aromas in the peel were basically the samewith those in the peel of‘Robertson’navel orange,however,the aromas in the pulp were different from those of two parents.

Key words: navel orange, graft chimaera, SSR, CpSSR, soluble sugar, organic acid, aroma

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