γ-射线照射梨试管苗诱导产生多倍体变异

园艺学报 ›› 2009, Vol. 36 ›› Issue (2): 257-260.

γ-射线照射梨试管苗诱导产生多倍体变异

孙清荣^1*;孙洪雁¹;祝恩元²;李林光¹

（¹山东省果树研究所，农业部泰安温带果树资源重点野外科学观测试验站，山东省果树生物技术育种重点实验室，山东泰安271000；²山东省科技厅，济南250014）

收稿日期:2008-08-06 修回日期:2008-12-10 出版日期:2009-02-25 发布日期:2009-02-25
通讯作者: 孙清荣

Polyploid Induction in Pear in vitro Treatment With Gamma-rays

SUN Qing-rong^1*,SUN Hong-yan¹,ZHU En-yuan², and LI Lin-guang¹

(¹Shandong Institute of Pomology, the State Agriculture Ministry Experiment Station of Temperate Fruits Germplasm Key Outdoor Observation in Taian, Shandong Fruits Biotechnology Breeding Key Lab, Tai’an 27100,China; ²Department of Science and Technology of Shandong Province, Jinan250014,China)

Received:2008-08-06 Revised:2008-12-10 Online:2009-02-25 Published:2009-02-25
Contact: SUN Qing-rong

摘要/Abstract

摘要：

用60Co γ-射线照射梨试管苗,照射后对其顶芽、侧芽和叶片3种外植体分别接种，经过继代和筛选，获得了形态变异明显的变异无性系，其变异表现为茎变粗，节间变短，叶长/宽比变小，叶柄变短。经染色体计数鉴定，这些变异中既有三倍体也有四倍体。多倍体的气孔长度及比叶重比对照二倍体显著增大。

关键词: 梨, 试管苗, γ-射线, 多倍体

Abstract: In vitro shoots of pear cultivar ‘Fertility’(Pyrus communis L.) were irradiated with gamma rays. Three different explants of shoot tip, lateral bud and young leaf from irradiated shoots were cultured separately. Shoots from 3 different explants were subcultured and selected. Morphological mutants with thicker stem, shorter internode, smaller leaf index and shorter petiole were obtained.Chromosome counting confirmed that the mutants were triploids and tetraploids. The lengths of stomatal guard cells and specific leaf mass of polyploids were significantly increased than that of diploids.

Key words: pear, in vitro shoots, gamma-rays, polyploid

中图分类号:

S 661.2

孙清荣;孙洪雁;祝恩元;李林光. γ-射线照射梨试管苗诱导产生多倍体变异[J]. 园艺学报, 2009, 36(2): 257-260.

SUN Qing-rong;SUN Hong-yan;ZHU En-yuan;and LI Lin-guang. Polyploid Induction in Pear in vitro Treatment With Gamma-rays [J]. ACTA HORTICULTURAE SINICA, 2009, 36(2): 257-260.

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