关键词: 桃, 遗传图谱, 雌蕊发育, 重瓣, 分子标记

Abstract: A linkage map of peach was constructed with SSR , AFLP and SRAP markers in 52 F₁ population derived from the cross of Prunus persica (L.) Batsch. Hongchuizhi and P. davidiana (Carr.) Franch. Baihuashanbitao. The selected primers including 38 SSR, 61 AFLP and 38 SRAP with rich polymorphism and steady bands were tested in progeny. 441 markers in 1:1 ratio by X2 test and 52 markers for double heterozygous loci were scored. Mapmaker software were used to assigned the markers in 1:1 ratio and pistil abortion trait to 11 linkage groups, the map is composed of 206 loci (18 SSRs, 126 AFLPs, 61 SRAPs, and one morphological trait) distributed in 11 linkage groups, the map covers 1193.2 cM of the peach genome. The average length of 11 groups is 108.5cM, the average distance between adjacent loci is 5.8 cM. Eighteen SSR loci in common with a saturated reference linkage map (T×E) of Prunus allowed a comparative analysis to be made between the two maps, homologies were found among the respective linkage groups, the map in this study showed better identity with peach reference map. Pistil development trait, were placed on the group1(Chromosome 1). Two AFLPs linked to a new double petal gene were obtained through analysis of the markers for double heterozygous loci.

Key words: Peach, Genetic linkage maps, Pistil development, Double petal, Molecule marker