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园艺学报 ›› 2014, Vol. 41 ›› Issue (3): 536-544.

• 研究报告 • 上一篇    下一篇

桃生长素反应因子和生长素/吲哚乙酸蛋白家族基因的克隆及表达分析

史梦雅1,张 巍1,余 佳1,王文平2,刘悦萍2,*   

  1. 1 北京农学院植物科学技术学院,北京 102206;2 北京农学院生物科学与工程学院,北京 102206
  • 出版日期:2014-03-25 发布日期:2014-03-25
  • 基金资助:

    国家自然科学基金项目(31101509)

Cloning and Expression Analysis of ARF and Aux/IAA Gene Family Members in Peach

SHI Meng-ya1,ZHANG Wei1,YU Jia1,WANG Wen-ping2,and LIU Yue-ping2,*   

  1. 1Plant Science and Technology College,Beijing University of Agriculture,Beijing 102206,China;2College of Biological
    Science and Engineering,Beijing University of Agriculture,Beijing 102206,China
  • Online:2014-03-25 Published:2014-03-25

摘要: 生长素对果实发育起着重要的调控作用。生长素反应因子(auxin response factor,ARF)和
生长素/吲哚乙酸蛋白(auxin/indoleacetic acids protein,Aux/IAA)是生长素信号转导系统中的两个关键因
子,在转录水平上对生长素参与的生理活动进行调控。为探索生长素调控桃果实发育的机制,选取ARF
家族的1 个基因PpARF1,Aux/IAA 家族的5 个基因PpIAA3、PpIAA9、PpIAA17、PpIAA26 和PpIAA29,
克隆其全长cDNA 序列并进行生物信息学分析,对它们在桃果实不同发育时期中果皮和种子的表达进行
qRT-PCR 检测。序列分析表明:这6 个基因的编码区全长分别为2 037、594、1 194、618、384 和723 bp,
分别编码678、197、397、205、127 和240 个氨基酸;氨基酸序列比对分析显示桃PpARF1 蛋白序列和
草莓的FvARF1(XP_004300014.1)同源性最高,达到90.56%,PpIAA 家族的5 个蛋白同源性很低,只
有23.77%;荧光定量PCR 结果显示,在花后52 d(果实发育硬核期),PpIAA3 和PpIAA17 在中果皮中的
表达量显著升高;PpIAA26、PpIAA29 和PpARF1 在种子中的表达量显著升高。预示生长素可能在桃果实
发育硬核期发挥着重要的调控作用。

关键词: 桃, 生长素反应因子, 生长素/吲哚乙酸蛋白, 基因克隆, 表达分析

Abstract: It has been known that auxin plays an important role in regulating fruit development. The
transcription factors ARF and repressor protein Aux/IAA were the key factors in auxin signal transduction
system,both of them are involved in regulating physiological activities at the transcriptional level. To
explore the role of auxin in regulating the expression of the target gene during peach fruit development,the
full-length of the cDNA sequences of the six related genes were isolated and analyzed by bioinformatics.
One of them was PpARF1 selected from ARF family and the other five were PpIAA3,PpIAA9,PpIAA17,
PpIAA26 and PpIAA29 selected from Aux/IAA family. The relative expression levels in mesocarp and seed of peach fruit at different developmental stages were also detected by quantitative real-time PCR. The
results showed that:The number of the base pairs of the six genes were 2 037,594,1 194,618,384 and
723 bp,which encoded six proteins containing 678,197,397,205,127 and 240 amino acids respectively.
The sequence of PpARF1 amino acid shares 90.56% homology with FvARF1(XP_004300014.1)in
Fragaria vesca;Homology of the five proteins belongs to the PpIAA family was very low,only 23.77%.
The expression of PpIAA3 and PpIAA17 were significantly increased in mesocarp at the 52 days after full
flowering(hard core period);The expression of PpIAA26,PpIAA29 and PpARF1 in seed also increased
significantly during that period. The results suggested that auxin might play an important role in regulating
development of peach fruit at hard core stage.

Key words: Prunus persica, ARF, Aux/IAA, cloning, expression analysis

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