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园艺学报 ›› 2014, Vol. 41 ›› Issue (3): 529-535.

• 研究报告 • 上一篇    下一篇

葡萄中盐诱导的R2R3-MYB 基因的筛选与表达分析

王春荣,王庆杰,李晓玲,姚玉新*,郝玉金*   

  1. 山东农业大学园艺科学与工程学院,作物生物学国家重点实验室,山东泰安 271018
  • 出版日期:2014-03-25 发布日期:2014-03-25
  • 基金资助:

    中国博士后科学基金项目(2011M500760);国家现代农业产业技术体系建设专项资金项目(CARS-30-ZP-06);山东省水果
    产业体系项目;教育部创新团队项目(IRT1155)

Screening and Expression Analysis of Salt-induced R2R3-MYB Genes in Grapevine

WANG Chun-rong,WANG Qing-jie,LI Xiao-ling,YAO Yu-xin*,and HAO Yu-jin*   

  1. College of Horticultural Science and Engineering, Shandong Agricultural University,State Key Laboratory of Crop
    Biology,Tai’an,Shandong 271018,China
  • Online:2014-03-25 Published:2014-03-25

摘要: 以葡萄砧木‘1103P’为试材,筛选获得了4 个受盐强烈诱导的R2R3-MYB 基因,系统进化
分析表明其编码蛋白属于不同的拟南芥R2R3-MYB 亚组。该4 个基因在根、茎、叶和果实中呈组成型表
达,但具有不同的表达水平,尤其是VvMYB112 在葡萄根系中具有较高的表达水平。在4 个基因中,
VvMYB112 能响应100 ~ 200 mmol · L-1 高盐处理。此外,VvMYB112 和VvMYB15 也能被聚乙二醇(PEG6000)
和低温诱导,而VvMYB107 和VvMYB87 对PEG6000 和低温不敏感或受其抑制。表明这4 个基因可能通
过不同的途径介导抗盐性。

关键词: 葡萄, R2R3-MYB, 转录因子, 盐, 基因表达

Abstract: In the study,we obtained four R2R3-MYB genes which were strongly induced by salt in the
roots of grapevine rootstock‘1103P’. The proteins encoded by the above four genes were clustered into
the different subgroups in the phylogenetic tree of Arabidopsis R2R3-MYB proteins. The four genes were
expressed in root,stem,leaf and berry,whereas they showed the different expression levels in the different
tissues. For example,VvMYB112 was expressed predominantly in the root. Among the four genes,only
VvMYB112 could be induced by 100–200 mmol · L-1 salt. Besides,VvMYB112 and VvMYB15 were
induced by PEG and cold as well,while VvMYB107 and VvMYB87 did not respond to or were repressed by
PEG6000 or cold. Taken together,the four screened genes showed the different expression patterns in the
different tissues or under the different stress treatment,which indicated that they might mediate the salt
resistance by the different pathways.

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