关键词: 灰毡毛忍冬, 快繁体系, 遗传稳定性, 分子鉴定

Abstract: An efficient mass propagation system of Lonicera macranthoides Hand.-Mazz. was
established based on axillary bud-derived meristems. The optimal media of tissue culture involving in the
bud-induction medium（MB medium supplemented with 1.0 mg · L^-1 6-BA，0.2 mg · L^-1NAA），subculture
medium（MB medium supplemented with 1.0 mg · L^-1 6-BA，0.5 mg · L^-1 IAA or 1.5 mg · L-1 6-BA，0.8
mg · L^-1 IAA）and root-induction medium（1/2MB medium supplemented with 2.0 mg · L^-1 IBA and 0.2
mg · L^-1 IAA）were selected. Thereafter，the genetic variation of 8 plants randomly sampled from
regeneration plants cultured for 12，24 and 36 months respectively was investigated by SRAP analysis and
there were 0，25% and 50% in variation rate found in regeneration lines respectively based on the 147
bands detected by SRAP markers. The results showed that regeneration culture system can maintain
genetic stability during certain subculture cycles，indicating that it can be readily employed for large-scale
propagation.

Key words: Lonicera macranthoides, rapid propagation system, genetic stability, molecular identification