https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2013, Vol. 40 ›› Issue (11): 2180-2188.

• 蔬菜 • 上一篇    下一篇

黄瓜CsFT 基因的克隆及其功能分析

张 娟,颜爽爽,赵文圣,张小兰*   

  1. 中国农业大学农学与生物技术学院蔬菜系,设施蔬菜生长发育调控北京市重点实验室,北京 100193
  • 出版日期:2013-11-25 发布日期:2013-11-25
  • 基金资助:

    中央高校基本科研业务费专项资金项目(2013RC030,2012RC012)

Cloning and Functional Analysis of CsFT in Cucumber

ZHANG Juan,YAN Shuang-shuang,ZHAO Wen-sheng,and ZHANG Xiao-lan*   

  1. Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops,College of Agronomy &
    Biotechnology,China Agricultural University,Beijing 100193,China
  • Online:2013-11-25 Published:2013-11-25

摘要: 利用同源克隆的方法得到黄瓜的FT 同源基因CsFT,通过表达分析、进化树分析以及遗传
转化拟南芥等方法初步验证了CsFT 在黄瓜花发育过程中的作用。不同于之前的研究结果,CsFT 主要在
雄花与雌花中表达,而在叶中基本不表达。异源过表达CsFT 导致拟南芥提前开花,并且茎顶部出现顶端
花,说明黄瓜的CsFT 基因与拟南芥AtFT 基因在开花调控上存在保守性。

关键词: 黄瓜, 开花, CsFT, 表达分析, 转基因植株

Abstract: Using homolog cloning,this paper identified the FT homolog in cucumber and explored the
functions of CsFT through expression analyses,phylogenetic characterization and ectopic transformation
in Arabidopsis. Distinct from previous studies,CsFT is highly expressed in male and female flowers,
whereas almost undetectable in the leaves. Overexpression of CsFT in Arabidopsis results in early
flowering and terminal flowers at top of shoots,indicating that CsFT may play conserved role during
flowering as that in Arabidopsis.

Key words: cucumber, flowering, CsFT, expression analysis, transgenic plant

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