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园艺学报 ›› 2013, Vol. 40 ›› Issue (11): 2171-2179.

• 蔬菜 • 上一篇    下一篇

辣椒种间(Capsicum annuum × C. frutescens)遗传图谱的构建与分析

周坤华1,2,陈学军1,*,方 荣1,陈丽珍3,宗洪霞1,4,缪南生1   

  1. 1 江西省农业科学院蔬菜花卉研究所,南昌 330200; 2 江西省农业科学院油料作物重点实验室,南昌 330200;3 江西省农业科学院,南昌 330200;4 江西农业大学农学院,南昌 330045
  • 出版日期:2013-11-25 发布日期:2013-11-25
  • 基金资助:

    国家自然科学基金项目(30860173,31060203)

Construction and Analysis of an Interspecific Linkage Map of Capsicum annuum × C. frutescens

ZHOU Kun-hua1,2,CHEN Xue-jun1,?,FANG Rong1,CHEN Li-zhen3,ZONG Hong-xia1,4,and MIAO Nan-sheng1   

  1. 1Vegetable and Flower Institute,Jiangxi Academy of Agricultural Sciences,Nanchang 330200,China;2The Key
    Laboratory of Oil Crop,Jiangxi Academy of Agricultural Sciences,Nanchang 330200,China;3Jiangxi Academy of
    Agricultural Sciences,Nanchang 330200,China;4College of Agriculture,Jiangxi Agricultural University,Nanchang
    330045,China
  • Online:2013-11-25 Published:2013-11-25

摘要: 以一年生辣椒(Capsicum annuum)B9431 为母本(P1),中国野生灌木辣椒(C. frutescens)
H108 为父本(P2)进行种间杂交,得到包含180 个单株的F2 作图群体,利用SRAP、SSR、ISSR 标记和
形态标记构建辣椒遗传图谱。该图谱共包含14 个连锁群,涉及264 个SRAP 标记、32 个SSR 标记和2
个ISSR 标记,控制软肉落果性状的S 基因定位于LG8。图谱总长1 023.45 cM,标记间平均图距3.42 cM。
每个连锁群的标记数在2 ~ 44 个之间,连锁群的长度在13.84 ~ 129.93 cM 范围内,平均图距在2.38 ~ 12.90
cM 之间。以SSR 标记为锚定标记,将该图谱与Minamiyama 等构建的图谱进行了初步对应。

关键词: 辣椒, 种间遗传图谱, SRAP, SSR, ISSR

Abstract: In this study,a population of F2 generation including 180 individual plants was derived from
the interspecific cross between the female line Capsicum annuum B9431 and the male line C. frutescens H108.
The SRAP,SSR,ISSR markers and morphological marker were used in constructing a genetic map. A
genetic linkage map containing 14 linkage groups(LGs)was constructed using 264 SRAP,32 SSR and 2
ISSR. The S gene controlling the soft flesh and deciduous fruit was located at LG8. The map covered 1 023.45
cM with an average marker interval of 3.42 cM. Number of markers included in linkage groups varied
from 2 to 44,mean marker interval distance from 2.38 cM to 12.90 cM and the length of linkage groups
from 13.84 cM to 129.93 cM individually. Furthermore,the links between the map published by
Minamiyama et al. and ours were established by the public microsatellites(SSR markers).

Key words: pepper, interspecific genetic map, SRAP, SSR, ISSR

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