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园艺学报 ›› 2009, Vol. 36 ›› Issue (1): 103-108.

• 研究简报 • 上一篇    下一篇

大白菜核基因雄性不育复等位基因Ms的SSR标记

冯 辉1*;魏 鹏1;李承彧1;崔水莲2;林容杓2;朴钟云1   

  1. (1 沈阳农业大学园艺学院,沈阳 110161;2 忠南大学基因组研究中心,韩国大田 305-764)
  • 收稿日期:2008-10-15 修回日期:2008-12-10 出版日期:2009-01-25 发布日期:2009-01-25
  • 通讯作者: 冯 辉

Identification of SSR Markers Linked to a Genic Multiple-Allele Male Sterile Gene

Feng Hui1*, Wei Peng1, Li Cheng-yu1, Choi Su Ryun2, Lim Yong Pyo2, and Piao Zhong-yun1   

  1. (1Department of Horticulture, Shenyang Agricultural University, Shenyang 110161; 2Plant Genome Research Center, Chungnam National University, Daejeon, 305-764, South Korea)
  • Received:2008-10-15 Revised:2008-12-10 Online:2009-01-25 Published:2009-01-25
  • Contact: Feng Hui

摘要:

利用大白菜细胞核复等位基因型雄性不育两用系‘AB01’的不育株,与多国芸薹属植物基因组计划MBGP的基础材料'Chiifu'杂交及回交,构建BC1群体。根据SSR检测体系中各主要成份浓度对扩增结果的影响,优化反应体系。选用250对SSR引物,对大白菜核基因雄性不育复等位基因Ms进行SSR+BSA分析,获得了7对多态性引物。在101株BC1个体间对这些引物进一步检测,得到2个与Ms基因连锁的SSR标记cnu_m273和cnu_m295。经连锁分析,二者位于Ms的同一侧,遗传距离分别为4.95 cM和7.92 cM。

关键词: 大白菜, 核基因雄性不育, 复等位基因, SSR

Abstract:

A BC1 mapping population was constructed with Chinese cabbage inbred line "Chiifu" which is the model material of Multinational Brassica Genome Project as the male parent and genic multiple allele male sterile material 'AB01' as the female parent. Major effects of components in SSR-PCR system on amplification result were probed. 250 SSR primer pairs were employed to identify SSR markers linked to the genic multiple allele male sterile gene "Ms". Within the eight SSR primer pairs showing polymorphism between two gene bulks, two SSR markers named cnu_m273 and cnu_m295 were found linked to the Ms gene, at the genetic distances of 4.95 cM and 7.92 cM respectively.

Key words: Chinese cabbage, male sterility, multiple allele, SSR

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