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园艺学报 ›› 2013, Vol. 40 ›› Issue (10): 1887-1896.

• 果树 • 上一篇    下一篇

套袋对‘茌梨’果实蔗糖代谢及相关酶基因表达的影响

杨绍兰,王 玫*,张晓菲,王成荣,王 然**   

  1. (青岛农业大学园艺学院,山东青岛 266109)
  • 出版日期:2013-10-25 发布日期:2013-10-25

Effects of Bagging Treatment on Sugar Metabolism and Related Gene Expression in‘Chili’Pear Fruits

YANG Shao-lan,WANG Mei*,ZHANG Xiao-fei,WANG Cheng-rong,and WANG Ran**   

  1. (College of Horticulture,Qingdao Agriculture University,Qingdao,Shandong 266109,China)
  • Online:2013-10-25 Published:2013-10-25

摘要: 研究了4种材质的果袋套袋处理对‘茌梨’(Pyrus bretschneideri Rehd.‘Chili’)果实生长发育过程中可溶性糖含量及蔗糖代谢的影响。结果表明,果实中的果糖、葡萄糖、蔗糖及可溶性总糖含量均随着果实的发育进程而增加;不同材质果袋影响糖增加的程度不同,套黄蜡纸袋的糖增加最少;转化酶(Ivr)活性随着果实的发育而降低,套袋降低了花后75 ~ 90 d 和花后150 d 果实酸性转化酶(AI)活性,而对中性转化酶(NI)影响不明显;蔗糖磷酸合成酶(SPS)活性随着果实的发育而增加,套袋处理不同程度上降低了SPS活性,以套黄蜡纸袋最为明显,其次是套白蜡纸袋和无纺布袋,套塑料膜袋的大部分测试点与对照差异不显著;随着果实的发育,果实中蔗糖合成酶合成活性(SSs)逐渐增加,蔗糖合成酶分解活性(SSc)逐渐下降,套袋对SSs活性影响在多数测定点差异不显著,但降低了果实发育后期SSc活性。蔗糖代谢相关酶基因AIV1AIV2SPS1SUS1表达的实时荧光定量PCR分析表明,套袋降低了AIV2的相对表达量,降低程度套无纺布袋大于套塑料膜袋;推迟了果实中SPS1的相对表达量最大值出现的时间,套塑料膜袋果的SPS1相对表达量在发育前期显著低于对照;影响了SUS1相对表达量的变化,整个发育过程未见套塑料膜袋果有SUS1相对表达量高峰出现,套黄蜡纸袋果的SUS1相对表达量最大值较对照提前15 d,之后显著低于对照。

关键词: 梨, 套袋, 糖, 代谢, 酶活性, 基因相对表达量, 实时荧光定量PCR

Abstract: Effects of different bagging treatments on the soluble sugar contents and sucrose metabolism of ‘Chili’(Pyrus bretschneideri Rehd.)fruit during growth and development were studied. The results showed that the contents of fructose,glucose,sucrose and total soluble sugar in the fruits increased with the fruit growth and development. Bagging,especially with yellow waxed paper bags,reduced the soluble sugar increases. Invertase(Ivr)activity decreased with fruit development,and bagging affected the acid invertase(AI)activity. AI activities in fruits of bagging treatments were lower than that in control in 75,90 and 150 d after flowering. The neutral invertase(NI)activity showed no significant difference between bagging treatments and control. Sucrose phosphate synthase(SPS)activity increased with the fruit development and affected markedly by bagging. Among 4 tested bags,the yellow waxed paper bags affected SPS activity of fruits more than the white waxed paper and non-woven fabric bags did,and there were not significant different between fruits in plastic film bags and non-bagging control in most of the tested points. Sucrose synthase(SUS)in synthesis activity(SSs)of fruits increased gradually and sucrose synthase in cleavage activity(SSc)decreased as fruit growth. Bagging did not showed significant effect on the SSs activity in most tested points,but depressed the SSc activity in fruit development late stage. Real-time fluorescent quantitative PCR analysis of sucrose metabolism related enzyme genes AIV1AIV2SPS1 and SUS1 expression showed that bagging reduced the relative expression of AIV2,and non-woven bags had more effects than plastic film bags. Bagging delayed the maximum accumulation time of SPS1,and the SPS1 relative expression were significantly lower in fruits with plastic film bags than in non-bagging control in the fruit growth early stage. Bagging altered the time course of SUS1 expression,the relative expression amount of SUS1 in fruits with plastic film bags did not reveal obvious peak,and in fruit with yellow waxed paper bags appeared a maximum accumulation 15 d earlier than control and became lower after.

Key words: pear, bagging, sugar, metabolism, enzyme activity, relative gene expression, real-time fluorescent quantitative PCR analysis

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