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园艺学报 ›› 2009, Vol. 36 ›› Issue (1): 33-38.

• 蔬菜 • 上一篇    下一篇

大白菜小孢子提取液中的蔗糖和甘露醇对胚胎发生的影响

李 菲;张淑江;章时蕃;孙日飞*   

  1. (中国农业科学院蔬菜花卉研究所, 北京 100081)
  • 收稿日期:2008-09-25 修回日期:2008-12-10 出版日期:2009-01-25 发布日期:2009-01-25
  • 通讯作者: 孙日飞

Effects of Sucrose and Mannitol in washing medium on microspore embryogenesis in Chinese cabbage [Brassica rapa L .ssp. pekinensis (Lour.) Olsson]

LI Fei,ZhANG Shu-jiang,ZhANG Shi-fan,and SUN Ri-fei*   

  1. (Institute of Vegetables and Flowers , Chinese Academy of Agricultural Sciences , Beijing 100081 , China)
  • Received:2008-09-25 Revised:2008-12-10 Online:2009-01-25 Published:2009-01-25
  • Contact: SUN Ri-fei

摘要:

研究了大白菜游离小孢子提取过程中,培养基中的蔗糖和甘露醇对后期小孢子胚胎发生的影响。结果表明在小孢子短期的提取过程中提高B5提取培养基的蔗糖浓度或添加甘露醇对小孢子的活力无影响,但会大幅度提高小孢子的胚胎诱导效果,其中17%蔗糖处理和8%甘露醇处理效果最佳,与对照相比平均出胚量都达到极显著水平,同时也促进了难诱导材料的胚胎发生。

关键词: 大白菜, 小孢子培养, 胚胎发生, 预处理, 蔗糖, 甘露醇

Abstract:

The effects of short-term pretreament were studied on embryogenesis of isolated microspores in Chinese cabbage. The results showed that B5 washing medium addition mannitol and with higher concentration of sucrose during isolation of microspores were not influence microspores viability but can significantly increased the frequency of embryogenesis . In our experiments, all nine cultivars responded to the treatment. B5 washing medium with 17% sucrose treatment and B5-13 washing medium with 8% mannitol treatment were the optimal treatment of two group tests .

Key words: Chinese cabbage, microspore culture, embryogenesis, pretreament, sucrose, mannitol

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