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园艺学报 ›› 2013, Vol. 40 ›› Issue (4): 767-.

• 研究报告 • 上一篇    下一篇

单倍体大白菜减数分裂分析及单体植株的获得

 刘富强, 代双艳*, 王彦华**?, 张成合, 申书兴   

  1. (河北农业大学园艺学院,河北保定 071001)
  • 出版日期:2013-04-25 发布日期:2013-04-25

Analysis of Meiotic Behavior of Haploid Chinese Cabbage and Selection of a Chromosome Monosomic Plant

LIU  Fu-Qiang, DAI  Shuang-Yan-*, WANG  Yan-Hua-**?, ZHANG  Cheng-He, SHEN  Shu-Xing   

  1. (College of Horticulture,Agricultural University of Hebei,Baoding,Hebei 071001,China)
  • Online:2013-04-25 Published:2013-04-25

摘要: 以大白菜小孢子培养获得的单倍体植株为材料,观察花粉母细胞减数分裂行为,测定花粉
生活力和单倍体植株杂交的结实性,并对子代植株的染色体进行了鉴定。结果表明:大白菜单倍体减数
分裂不规则,中期Ⅰ单价体游离于赤道板附近或排列在赤道板上,可见1 ~ 2 个局部联会的二价体或1 个
局部联会的三价体;后期Ⅰ单价体大多能够移向两极,包含0/10、1/9、2/8、3/7、4/6 和5/5 等分离方式,
少数移向三极,包含1/4/5 和2/2/6 等分离方式;减数分裂产物包含二分孢子、三分孢子和四分孢子等;
单倍体植株与二倍体植株杂交表现高度不育,平均每荚结籽仅为0.068 粒;11 个子代植株中10 株为二倍
体(2n = 20),1 株为单体(2n–1 = 19)。

关键词: 大白菜, 单倍体, 减数分裂, 单体植株

Abstract: Meiotic behavior and pollen viability of the haploid Chinese cabbage derived from
microspore culture were studied and the chromosome numbers of progeny plants of the haploid plants
crossing to the diploid plants were examined in the present report. The results showed the meiotic behavior
of the haploid Chinese cabbage was abnormal. At metaphaseⅠ,the univalents were either moved to the
center of the cell(equatorial plane)or scattered in the cytoplasm,occasionally 1–2 bivalents or one
trivalent occurred;At anaphase Ⅰ,the chromosomes freely went to opposite poles,resulting in 0/10,
1/9, 2/8,3/7,4/6 and 5/5 chromosome distributions,occasionally to three poles,forming 1/4/5,2/2/6
and so on chromosome distributions. The products of the meiosis included tetrads,dyads and triads. The
seed set of the haploid plants × the diploid plants by artificial pollination was very low,only about 0.068
seeds per pod. By chromosome number identification,of the total 11 progeny plants,ten plants were
diploid (2n = 20)and one monsomic plant(2n–1 = 19).

Key words: Chinese cabbage, haploid, meiosis, monosomic plant

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