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园艺学报 ›› 2013, Vol. 40 ›› Issue (4): 685-.

• 蔬菜 • 上一篇    下一篇

苦瓜枯萎病抗性鉴定与抗性遗传规律研究

 赵秀娟, 唐 鑫, 胡开林   

  1. (1 广东科贸职业学院,广州 510430;2 华南农业大学园艺学院,广州 510642)
  • 出版日期:2013-04-25 发布日期:2013-04-25

Studies on the Identification Technology and Inheritance of Disease Resistance to Fusarium Wilt in Bitter Gourd

 ZHAO  Xiu-Juan, TANG   Xin, HU  Kai-Lin   

  1. (1Guangdong Science and Tradition College of Vocation,Guangzhou 510430,China;2College of Horticulture,South
    China Agricultural University,Guangzhou 510642,China)
  • Online:2013-04-25 Published:2013-04-25

摘要: 以4 份田间抗性水平不同的苦瓜为材料,探讨了适用于苦瓜苗期人工接种枯萎病菌的抗性
鉴定方法;以此为基础,对来自国内外的43 份苦瓜种质资源进行了抗源筛选,以其中的抗病亲本‘0417’
和感病亲本‘472113’为材料,研究了苦瓜对枯萎病抗性的遗传规律。结果表明,直接水培接种法是较适
合于苦瓜苗期枯萎病抗性鉴定的方法,适宜的接种菌液孢子浓度为4 × 106 · mL-1。在苦瓜种质资源中,枯
萎病抗源普遍存在,尤以野生种或半栽培种抗病性较强。苦瓜枯萎病抗性受单一显性核基因控制,其广
义遗传力为90.78%。

关键词: 苦瓜, 枯萎病, 苗期接种, 抗性遗传

Abstract: The identification technology of resistance to Fusarium wilt in Momordica charantia was
investigated with inbred lines presenting different degree of field resistance. Then a total of 43 Momordica
charantia varieties(or inbred lines)from home and abroad were evaluated for resistance to Fusarium wilt.
Furthermore,a highly susceptible inbred line‘472113’and a highly resistant inbred line‘0417’were used
to study the inheritance of resistance to Fusarium wilt in Momordica charantia. The results indicated that
direct hydroponic inoculation with concentration of 4 × 106 spores per milliliter was most suitable for
resistance identification. And the existence of resistant resource is prevalent among the Momordica
charantia germplasm resources. The broad-sense heritability of Momordica charantia resistance to
Fusarium wilt is 90.78%. The resistance is controlled by single dominant nuclear gene without affecting
by cytoplasm.

Key words: bitter gourd, Fusarium oxysporum f. sp. momordicae Sun &, Huang, seedling inoculation, resistance inheritance

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