云南澳洲坚果苗木感染番茄斑萎病毒属病毒初报

园艺学报 ›› 2013, Vol. 40 ›› Issue (2): 350-354.

云南澳洲坚果苗木感染番茄斑萎病毒属病毒初报

方琦, 丁铭, 董家红, 尹跃艳, 张磊, 苏晓霞, 李婷婷

（1 云南省农业科学院生物技术与种质资源研究所，昆明 650223；2 云南省农业生物技术重点实验室，昆明 650223）

出版日期:2013-02-25 发布日期:2013-02-25

Preliminary Report of Tospovirus Infecting Macadamia Seedling in
Yunnan，China

FANG Qi, DING Ming, DONG Jia-Hong, YIN Yue-Yan, ZHANG Lei, SU Xiao-Xia, LI Ting-Ting

（1 Institute of Biotechnology and Genetic Resources，Yunnan Academy of Agricultural Sciences，Kunming 650223，China；
2 Key Laboratory of Agricultural Biotechnology of Yunnan Province，Kunming 650223，China）

Online:2013-02-25 Published:2013-02-25

摘要/Abstract

摘要： 在云南省临沧市永德县海拔约800 m 的澳洲坚果（Macadamia sp.）种苗繁育基地发现叶片
褪绿、黄化、叶脉间呈现近似圆形或不规则褐色枯斑的植株。为了确认植株病因，采集典型病株，应用
电子显微镜负染色法、超薄切片和DAS-ELISA 进行检测，在澳洲坚果苗木病叶分离物Maca-LC 的粗汁
液和病叶超薄切片中均观察到有类似番茄斑萎病毒属（Tospovirus）病毒粒体。该病毒粒子为球形，直径
80 ~ 85 nm。进一步应用DAS-ELISA 方法鉴定，病叶与Tospovirus 的西瓜银色斑驳病毒（Watermelon silver
mottle virus，WSMoV）/花生芽坏死病毒（Groundnut bud necrosis virus，GBNV）复合抗血清呈阳性反应。
通过病症典型症状、病毒形态学观察及血清学方法检测表明，侵染云南澳洲坚果种苗的病毒分离物
Maca-LC 为番茄斑萎病毒属Tospovirus 病毒。

关键词: 澳洲坚果, 番茄斑萎病毒属, 超薄切片, DAS-ELISA

Abstract: Infected plant seedlings of macadamia showing concentric chlorotic ring spot or brown
lethal spot symptoms were found in a nursing base located at the altitude of about 800 meters in Lincang，
Yunnan，China. In order to confirm the presence of virus，typically infected leaves of Macadamia sp. were
detected by using electron microscopy and DAS-ELISA. Virions with a diameter of about 80–85 nm，
similar in size to the Tospovirus particles，were both observed in the sap and ultrathin sections of diseased
leaves of macadamia. The crude extracts of infected leaves had a positive reaction to the complex antibody
of Watermelon silver mottle virus（WSMoV）/Groundnut bud necrosis virus（GBNV）. These results above
indicated that macadamia was infected by a Tospovirus. It is the first report of Tospovirus infecting macadamia.

Key words: macadamia, Tospovirus, ultrathin section, DAS-ELISA

中图分类号:

S 667.9

方琦, 丁铭, 董家红, 尹跃艳, 张磊, 苏晓霞, 李婷婷. 云南澳洲坚果苗木感染番茄斑萎病毒属病毒初报[J]. 园艺学报, 2013, 40(2): 350-354.

FANG Qi, DING Ming, DONG Jia-Hong, YIN Yue-Yan, ZHANG Lei, SU Xiao-Xia, LI Ting-Ting. Preliminary Report of Tospovirus Infecting Macadamia Seedling in
Yunnan，China[J]. ACTA HORTICULTURAE SINICA, 2013, 40(2): 350-354.

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