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园艺学报 ›› 2013, Vol. 40 ›› Issue (2): 221-230.

• 果树 • 上一篇    下一篇

尿素对葡萄5 个氮代谢相关基因表达的影响

 初建青, 岳林旭, 房经贵, 刘 洪, 宋长年, 张演义   

  1. (1 南京农业大学园艺学院,江苏省果树品种改良与种苗繁育工程中心,南京 210095;2 山东省轻工农副原料研究所,
    山东高密 261500;3 江苏省农业科学院园艺研究所,南京 210014;4 聊城大学农学院,山东聊城 252059)
  • 出版日期:2013-02-25 发布日期:2013-02-25

Effects of Fertilizer Application on Expression of Genes Related to
Nitrogen Metabolism in Fujiminori Grapevine

CHU  Jian-Qing, YUE  Lin-Xu, FANG  Jing-Gui, LIU   Hong, SONG  Chang-Nian, ZHANG  Yan-Yi   

  1. (1College of Horticulture,Nanjing Agricultural University;Jiangsu Fruit Crop Genetics Improvement and Seeding
    propagation Engineering Center,Nanjing 210095,China;2Institute of Agricultural and Sideline Production Material,
    Shandong Light Industry Department,Gaomi,Shandong 261500,China;3Institute of Horticulture,Jiangsu Academy of
    Agricultural Sciences,Nanjing 210014,China;4College of Agronomy,Liaocheng University,Liaocheng,Shangdong
    252059,China)
  • Online:2013-02-25 Published:2013-02-25

摘要: 以‘藤稔’葡萄为试材,利用半定量RT-PCR 和荧光定量PCR 技术对5 个葡萄氮代谢基因
VvGHD(JF796045)、VvNiR(JF796046)、VvNR(JF796047)、VvGS(JF796048)和VvAS(JF796049)
在施用尿素后的表达情况进行了分析。5 个基因在葡萄叶面喷施尿素后不同时间段的表达量存在显著差
异,整体呈先升高后降低的趋势;VvGDH 最高表达水平发生在尿素喷施后48 h,VvNRVvNiR 最高表
达水平发生在24 h,VvGS 最高表达水平发生在6 h,VvAS 最高表达水平发生在2 h。叶面喷施比土壤施用
尿素叶中5 个基因表达高峰出现的时间早,且表达水平高;土壤施尿素后叶中5 个基因对尿素的响应时
间比根中延后,但响应强度明显高于根中。

关键词: 葡萄, 氮代谢, 基因, 表达

Abstract: To understand gene response to fertilizer application,quantitative PCR and semiquantitative
PCR were used to analyze the expression of five genes(VvGHDVvNiRVvNRVvGS and
VvAS)of‘Fujiminori’grape at different times with the fertilizer application. The results show that the
expression levels of five genes exhibit significant differences at different times after treatment,the overall
trend was first increased and then decreased;The highest expression level of VvGDH was in 48 h,VvNR
and VvNiR the highest expression level in 24 h,VvGS the highest expression level in 6 h,VvAS the highest
expression level in 2 h. The highest expression levels of five genes after ground fertilization in leaves were
delayed longer than those after foliar fertilizer application,and the overall expression level lower than
foliar fertilizer application;Response of five genes after ground fertilization in leaves was delayed longer
than the root,but the response intensity was significantly higher than corresponding genes in the root.

Key words: grapevine, nitrogen metabolism, gene clone, gene expression

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