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园艺学报 ›› 2012, Vol. 39 ›› Issue (12): 2341-2351.

• 果树 •    下一篇

干旱对葡萄果实发育过程中黄烷醇类多酚积累及LAR 表达的影响

温鹏飞, 杨运良, 高美英, 牛铁泉, 邢延富, 牛兴艳   

  1. (山西农业大学园艺学院,山西太谷 030801)
  • 出版日期:2012-12-25 发布日期:2012-12-25

The Effect of Drought on Flavanols Accumulation and LAR Expression During the Development of Grape Berry

WEN  Peng-Fei, YANG  Yun-Liang, GAO  Mei-Ying, NIU  Tie-Quan, XING  Yan-Fu, NIU  Xing-Yan   

  1. (College of Horticulture,Shanxi Agricultural University,Taigu,Shanxi 030801,China)
  • Online:2012-12-25 Published:2012-12-25

摘要: 以酿酒葡萄‘赤霞珠’(Vitis vinifera L.‘Cabernet Sauvignon’)为试材,采用避雨棚和断根沟措施,模拟土壤干旱,探讨了葡萄果实发育过程中干旱对果实中黄烷醇类多酚积累及其合成关键酶隐色花色素还原酶(leucoanthocyanidin reductase,LAR)基因表达的影响。结果表明:土壤干旱导致葡萄单粒质量降低,纵、横径减小,促进黄烷醇类多酚积累,特别是在幼果期,土壤干旱明显促进黄烷醇类多酚的积累。Real-time PCR、Western blot结果证实,土壤干旱促进VvLAR1VvLAR2转录,诱导果实中LAR1、LAR2新蛋白合成,进而导致LAR酶活性增强,黄烷醇类多酚积累。

关键词: 葡萄, 果实, 土壤干旱, 黄烷醇类多酚, LAR

Abstract: In order to understand the effects of soil drought on the accumulation of flavanols and the expression of leucoanthocyanidin reductase(LAR),the key enzyme of the flavanols biosynthesis in flavonoid biosynthesis pathway,5 years old grapevines(Vitis vinifera L.‘Cabernet Sauvignon’)were used as materials,and the soil drought was simulated during grape berry development by the controlling irrigation through the prevent-rain shelter and root-cutting groove. The results showed that the single berry weight,longitudinal and horizontal diameter were decrease,and the growth of grape berry was significantly inhibited by soil drought,which indicated that the prevent-rain shelter and root cutting groove were the effective measures to simulate soil drought. In addition,the accumulation of flavanols in grape berry during its development was induced by soil drought was also observed,especially in the young berry. Real-time PCR and Western blot results showed that the soil drought could induce the expression of VvLAR1,VvLAR2,the synthesis of LAR new protein,and increased the LAR enzyme activity,which resulted in the accumulation of flavanols in grape berry.

Key words: grape, berry, soil drought, flavanols, LAR

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